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EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN:9781305446021
Author:Lampman
Publisher:Lampman
Chapter89: Thin-layer Chromatography
Section: Chapter Questions
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Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Thank you for your help on these questions!

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Transcribed Image Text:solvent front origin
15. Consider the following plate of compounds, A, B, and C developed in Hexanes:
a. Determine the Rf values of compounds A, B, C run on a silica gel TLC plate using hexanes as solvent
2. Which compound, A, B, or C is most polar?
3. What would you expect to happen to Rf values if you used acetone instead of hexanes as the eluting solvent
Separation of Amino Acids by Thin Layer Chromatography
4. How would the Revalues change if the eluted with hexanes using alumina TLC plates?
Transcribed Image Text:15. Consider the following plate of compounds, A, B, and C developed in Hexanes: a. Determine the Rf values of compounds A, B, C run on a silica gel TLC plate using hexanes as solvent 2. Which compound, A, B, or C is most polar? 3. What would you expect to happen to Rf values if you used acetone instead of hexanes as the eluting solvent Separation of Amino Acids by Thin Layer Chromatography 4. How would the Revalues change if the eluted with hexanes using alumina TLC plates?
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