whenanalyzing urine from a patient with kidney disease which organic molecules might be detected which biochemical test will be the most appropriate for detectionof those molecues?
Q: You have obtained a 10X fold purification and a yield of 10% at the Affinity step. What was the…
A: Given, Specific activity (SA) at affinity step = 20833 U/mg Total activity (TA) at affinity step =…
Q: The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater sensitivity compared to other…
A:
Q: Calculate the cocentrations of acetic acid (pKa = 4.76) and sodium acetate necessary to prepare a…
A:
Q: Many of the buffers used these days, such asHEPES and PIPES, were developed because they have…
A: A buffer is a solution that prevents the pH of a sample from decreasing or increasing when acids or…
Q: • Outline the biosynthesis of tuberculostearic acid using phospholipid-bounded oleic acid and SAM as…
A: Tuberculostearic acid is a fatty acid found in the cell wall of Mycobacterium. The fatty acid was…
Q: Explain amino acids being amphoteric electrolytes to form peptide bonds. State at least 5 general…
A: Biuret test is also known as Piotrowskis’ test. This test is used to determine the presence of…
Q: . A protein gives, under conditions of buffer composition, pH, and tem- perature that are close to…
A: Given values: The given molecular weight of the native protein (Under physiological conditions) =…
Q: A biochemist wants to separate two peptides by ion-exchange chromatography. At the pH of the mobile…
A: A biochemist wants to separate 2 peptides by the ion-exchange chromatography. One peptide has a…
Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…
A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…
Q: if glycogen is subjected directly to nelson's assay without prior hydrolysis
A: Nelson's assay method or Nelson-Somogyi method is used for the quantitative determination of…
Q: mixture and what the expected resulted would be bas on the specific procedure you have used.…
A: A polypeptide is made up of an amino acid chain.
Q: 1 TC: 200 250 300 350 Wavelength (nm) UV/Vis spectrum of four brands of polysorbate 80, a common…
A: Coomassie dye (Blue G-250; also refer as CBB) binds to protein and gives a colorimetric change in…
Q: Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 Time (mins) -…
A: Ampicillin is an antibiotic which inhibits the bacterial enzyme transpeptidase by binding to it…
Q: What is the [CH3COO−]/[CH3COOH] ratio inan acetate buffer at pH 4.00?
A: A buffer is a mixture of weak acid/weak base and its conjugate base/acid. It is an aqueous solution.…
Q: Name at least three test reactions for lipids. Briefly explain the the principle of each test and…
A: A group of organic compounds includes lipids that are insoluble or poorly soluble in…
Q: Mention assays other than biuret and lowry assays that are used to detect the presence of proteins.…
A: Introduction: Protein is the major macromolecule that plays a vital role in the structure and…
Q: Acid hydrolyses of proteins is often used to determine the amino acid composition of a protein.…
A: Proteins are made up of small units or building blocks. These units are called amino acids.…
Q: A mixture containing glutamic acid, arginine, phenylalanine and valine was subjected to anion…
A: Amino acids differ from each other on the basis of their side-chain properties. There are twenty…
Q: Based on the experimental data provided, estimate the amount of amino acid in the given unknown…
A: Background information Ninhydrin is a strong oxidizing agent. It causes oxidative decarboxylation…
Q: ttheendoftheprocedureforthisexperiment,arecrystallizationisdoneina mixed solvent system. Which…
A: Recrystallization is generally happens using a mixture of the solvent of two solvents is polar…
Q: (2) Tris buffer is an organic buffer that is commonly used in Biochemistry experiments in lieu of…
A: Buffers are solutions that resist changes in pH in a solution. The pH buffering range of any buffer…
Q: During successufl purification of every enzyme, the following may be expected: 1. Solubility in NaCl…
A: The specificity increases during the purification of every enzyme.
Q: The-carotene molecule has λmax 450 nm and ɛ = 15.000 m2 / mol. Calculate the expected absorbance…
A: Absorbance is the amount of light absorbed by a solution. It is also termed optical density.…
Q: how/why you will characterize the proteins (whether impurities or dsRed.m1) as it comes off the DEAE…
A: Diethylaminoethyl cellulose (DEAE-C) It is a type of resin that is Hydrophilic, Positively charged,…
Q: Qualitative Analysis of Carbohydrates Carbohydrates undergo dehydration by Conc. H2SO4 to form…
A: A biomolecule is a molecule that is produced by cells or living creatures. Carbohydrates, proteins,…
Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…
A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…
Q: Why is the absorbance spectrum of the iron (II) complex obtained first prior to measuring the…
A: Colorimetric analysis is based on how the intensity of a solution's colour changes as its…
Q: You need a buff er at pH 7.5 for use in purifying a protein at 4°C. You have chosen Tris, pK 8.08,…
A: pH affected by a change in temperature.
Q: Salting out and gelation of proteins is most advisable to carry out when biopolymers are in the…
A: Proteins are biomolecules made up of different amino acids. A protein becomes completely active in…
Q: In solubility test, why do we need to put the lipid samples in a direct heat after adding ethanol?
A: In solubility test, why do we need to put the lipid samples in a direct heat after adding ethanol?…
Q: ole of nitrite ion and thiosulfate ion in treatment of cyanide Poisoning
A: Cyanide is a chemical group containing cyano group, which is C=N. Exposure to several forms of…
Q: Electrospray can transfer the peptide from solution into the gas-phase while retaining the peptides…
A: Peptides are short chains of amino acids linked by peptide/amide bonds. The property of the peptide…
Q: Write the biochemical structures of bicarbonate buffer Phosphate buffer and protein buffer.…
A: Bicarbonate buffer system It is used to balance pH in blood and duodenum along with some other…
Q: An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by…
A: In reverse-phase HPLC with a non polar stationary phase and a polar solvent as the mobile phase at…
Q: briefly describe the disadvantages of acid hydrolysis of proteins method.
A: Protein is a polypeptide molecule comprised of amino acids. Protein is synthesized in the cytoplasm…
Q: Why are buffers important in biochemical experiments? Please explainw ell
A: Buffer : solution which resists any change in pH via the addition of any acidic/ the basic…
Q: Give two advantages and two disadvantages to using the biuret reaction to measure protein…
A: Aromatic amino acids such as tyrosine, tryptophan and phenylalanine exhibit strong UV-light…
Q: Sketch a titration curve for aspartic acid, andindicate the pKa values of all titratable groups.…
A: To calculate the pH range when the conjugate acid-base pair +1 Asp and 0 Asp will act as a buffer,…
Q: Can you recommend the most suitable method for purification of peptides in acidic solution…
A: Introduction: Peptides are the short chain of amino acids connected by peptide bonds. It consists of…
Q: Explain why lipids are insoluble in polar solvents. How do oils and fats differ? How does the degree…
A: Hi! We are authorized to author a maximum of 3 subparts at a time. Since its not mentioned which…
Q: Define and give description of the importance of the isoelectric precipitation of proteins. (Hint:…
A: Proteins are large molecules consisting of one or more long chains of amino acid residues. Proteins…
Q: Downstream processing will primarily be concerned with initial separation of the bioreactor broth…
A: Downstream processing is a sequence of events that include separation of the cell, filtration, and…
Q: Cyanide ion (CN-) causes toxicity to humans, explain the reason of its toxicity to us. Suggest a…
A: Cyanide is a compound that belongs to the cyano group. In this chemical compound, carbon is bonded…
Q: An import relationship that allows us to determine the pKa of a weak acid using a pH titration curve…
A: The Henderson-Hasselbalch equation gives the relation between pH and pKa. pH defines the…
Q: Based on the intermediates outlined below, suggest why in different solvents and at different pH…
A: Carbonyl compounds are organic compounds with aldehyde or ketone as the functional group. It…
Q: ion required with these question: What are the purpose of pharmac
A: Pharmaceutical products are medicines or drugs that are used to produce a biological effect when…
Q: Determine the net charge of the predominant form of Asp at (c) pH 6.0, and (d) pH 11.0.
A: Introduction- Aspartic acid is an alpha non-essential amino acid that is occurring naturally in the…
Q: Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest…
A: Ion exchange chromatography is used to separate the charged molecules such as amino acids and…
Q: The tests for the hydrolysis of starch, casein, and triglycerides are similar in terms of setup. How…
A: Hydrolysis is the chemical reaction in which a water molecule is involved in rupturing one or more…
Q: An appropriate biochemical buffer should have charges in both conjugate acid and conjugate base…
A: Buffer solutions often are weak acid/strong acid and conjugate base. buffers resist the change…
whenanalyzing urine from a patient with kidney disease which organic molecules might be detected which biochemical test will be the most appropriate for detectionof those molecues?
Kidney Disease means that the Kidneys are not functional and unable to filter the blood. Kidney Disease usually results from hypertension and diabetes that is high blood sugar.
Step by step
Solved in 2 steps
- Which organic molecules may be found in urine from a patient with kidney illness, and which biochemical test would be the most suited for detecting those molecues?When analyzing urine from a patient with kidney disease, which organicmolecules might be detected? Explain. Which biochemical test will be the most appropriate for detection of those molecules?Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____
- Give two advantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.When comparing the biuret reaction to measuring protein absorbance directly at 280 nm, list two benefits of utilizing the biuret reaction to quantify protein concentration.give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.
- A mixture containing glutamic acid, arginine, phenylalanine and valine was subjected to anion exchange chromatography at pH 7.0. Which of the amino acids interacted with the resin (anion exchanger) and last to be eluted with a buffer of decreasing pH? A. Glu B. Val C. Phe D. ArgGive two advantages and two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nmDescribe the principles involved in the separation of proteins by sodium dodecyle sulphate (SDS) PAGE and isoelectric focusing.
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine’s test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.For the determination of total lipid content in food samples which matrix effects are expected to be significant, suggest an appropriate method with reasons and outline the corresponding procedures.briefly describe the disadvantages of acid hydrolysis of proteins method.