The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater  sensitivity compared to other conventional spectroscopy techniques. Explain  the underlying mechanism of this.

Q: A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their…

A: Beer Lamberts law states that ;  A=ε CIwhere 'A' is absorbance; 'ε' is molar absorptivity…

Q: What are the processes of concentrate samples analysis in Uv-visible spectroscopy?

A: UV-visible spectroscopy is a fast and simple technique that finds the analyte concentration of a…

Q: Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).

A: Introduction: Surface-enhanced Raman spectroscopy (SERS) is a strong microscopic observation…

Q: The following Rf values were computed from a reverse phase chromatography experiment. Arran them…

A: There are different techniques to separate a compound from a mixture of substances. The different…

Q: Explain the occurrence in which isolated peaks are formed in a characteristic x-ray spectra result.

A: X ray spectra: the spectrum that is obtained by emission of x rays which are diffracted by a crystal…

Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…

A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to…

A: This is the structure of Sulfamethoxazole.

Q: The two most abundant pigments in the chromatography results were carotene and Xanthophyll. What…

A: A photosynthetic pigment is a pigment which is found in green plants and do not  absorb all the…

Q: Why might Gamma irradiation be considered better than using UV radiation?

A: It is required to provide reason that Gamma irradiation is considered better than using UV radiation…

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: Write shortly about the instrumentation of ATR spectroscopy?  Answer should be to the point.

A: ATR(Attenuated Total Reflectance), is the sampling technique used in infrared spectroscopy (IR) to…

Q: phenazopyridine

A: Phenazopyridine could be a pain reliever that affects the lower a part of your tract (bladder and…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: Explain the purpose of the Kirby-Bauer diffusion test?

A: Kirby-Bauer diffusion test/Disk diffusion method It is most widely used antibiotic susceptibility…

Q: Describe in short the process of size exclusion chromatography (molecular exclusion chromatography)

A: Size exclusion chromatography is a separation of biomolecules techniques where separation was done…

Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…

A: Spectrophotometric analysis has been widely used in biomedical science to determine the…

Q: Define iodide pump.

A: Iodide pump : An integral membrane protein that co transports Na+ and I- into the thyroid follicles.…

Q: Briefly describe the difference between chromatography and ion exchange

A: Asked : Difference between chromatography and ion exchange

Q: Briefly explain the principle behind the following techniques: (a) ion-exchange chromatography  (b)…

A: Chromatography is a biophysical method for separating compounds from a mixture. It works on the…

Q: why steroids do not show luminescence on thin layer chromatographic plates?

A: Infrared absorption spectroscopy also stated as IR spectroscopy is one of the most valuable…

Q: State the principle that underlies the following biochemical tests: a) Methyl red test b)…

A: Please note that as per the guidelines of Bartleby, only question no. 1 with both subparts (a) and…

Q: What is the role of imidazole in Ni-Affinity Chromatography?

A: Chromatography is the technique where the mixture of proteins gets separated based on various…

Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…

A: Column chromatography is a purification method that uses the polarity of compounds for the…

Q: Compare AND contrast an intercalator and an alkylating agent.

A: Intercalating agents and alkylating agents, both have effects on the cell's DNA by altering it.

Q: What is the importance of normalization of data in fluorescence spectroscopy

A: Introduction :- An electromagnetic spectroscopy technique called fluorescence spectroscopy examines…

Q: Chromatography technique works on the principle of

A: Chromatography is a technique used for separation of mixture by using a moving solvent.

Q: the principle behind this technique; affinity chromatography

A: Chromatography is a technique of separation of components of any mixture dissolved in a dynamic…

Q: Justify and explain following statements.  (a) Paper chromatography is a type of partition…

A: Chromatography is a technique of separation which is used for separating the components present in a…

Q: State the principle that underlies the following biochemical tests: a) Methyl red test b)…

A: (a) Methyl Red Test Principle These bacteria initially metabolize glucose to pyruvic acid, which is…

Q: What is the importance of sllid width in fluorescence spectroscopy

A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.

Q: How does HPLC differ from ionexchange chromatography?

A: In chromatography, mixture of compounds are separated into individual components based on their…

Q: What is the working principle of raman spectroscopy.

A: Raman spectroscopy helps in determining both qualitative and quantitative information of the analyte…

Q: Column Chromatography Another name for separation in column chromatography is

A: Another name for seperation in coloumn chromatography is Adsorption column chromatography.…

Q: Different light source requires for the molecular absorption spectrometer and for an atomic…

A: A spectrometer is any instrument used to measure change in spectrum. This could be a mass-to-charge…

Q: List two situations when you would not be able to use the UV-Vis absorbance at 280 nm to determine a…

A: There are different ways of determining protein concentration. UV-Vis spectroscopy is one such…

Q: State the principle that underlies the following biochemical tests: a. Voges-Proskauer test

A: Voges and Proskauer, in 1898, first noticed the creation of a red tone after the addition of…

Q: What is doping?   Explain the mechanism of mass spectrometry.

A: In semiconductor production: Doping is the intentional introduction of impurities into an intrinsic…

Q: Discuss briefly Mössbauer spectroscopy. the principle of

A: Spectroscopy is the study concerned with the measurement of spectra produced when a substance…

Q: Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV…

A: Sulfamethoxazole Sulfamethoxazole is a sulfonamide based bacteriostatic antibiotic, widely used to…

Q: Which of the following scientists use this X-ray crystallography?

A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…

Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…

A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…

Q: Dilute silver nitrate is sometimes applied to the eyes of newborn infants. Explain why a dilute…

A: Eye is an organ which helps us to provide vision it has several photoreceptors which receives light…

Q: What is the basic purpose of the paper chromatography?

A: Chromatography is an important analytical method used to separate a mixture of chemical substances…

Q: How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at…

A: Fluorescence spectroscopy uses a beam of light that excites the electrons that are present in the…

Q: Explain the differences, if any, between adsorption and gas solid chromatography

A: Introduction- Chromatography is a technique that is used to separate the mixtures present in the…