Give two advantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.
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Give two advantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.
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- give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.Give two advantages and two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nmGive two benefits of utilizing the biuret reaction to assess protein concentration over directly measuring protein absorbance at 280 nm.
- When comparing the biuret reaction to measuring protein absorbance directly at 280 nm, list two benefits of utilizing the biuret reaction to quantify protein concentration.What is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm with the presence of the Bradford reagent? (note: explain not less than 5 sentences).In quantitative determination of protein using spectrophotometer how can I plot the absorbance vs wavelength for the proteins given the transmittance values?
- Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.topic: Determination of Protein Concentration by Spectrophotometry Enumerate and discuss the limitations of Beer-Lambert’s Law.
- When analyzing urine from a patient with kidney disease, which organicmolecules might be detected? Explain. Which biochemical test will be the most appropriate for detection of those molecules?Calculate the concentration of 0.2ml amino acid when path length is 1cm and has an absorbance value of 0.50, if a 0.5ml solution of same amino acid has a concentration of 0.72mg/ml with an absorbance of 0.9.In the Biuret Assay for protein concentration determination, the role of sodium potassium tartrate is to prevent the (gaining, losing) of electrons by Cu2+.