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Give typing answer with explanation and conclusion
Which antigen was returned to the 901 series because there was no determined linkage to the SMIM1 gene?
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- Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control ACell line A was cultured in the absence and presence of KB9520 or PPT. You then lyse the cells and fractionate them, generating membrane, cytosolic and nuclear protein fractions of the whole cell lysate. Western blots were then run using antibodies against ER (alpha) and ER (beta) for each treatment, and include different lanes for the membrane, cytosolic and nuclear protein fractions. Draw the expected results for these western blots and briefly discuss the expected differences."The antibody diversity created by the combinatorial joining of V, D, and J segments by V(D)J recombination pales in comparison to the enormous diversity created by the random gain and loss of nucleotides at V, D, and J joining sites", is true or false.
- The immunoglobulin molecules of a particular mammalian species have kappa and lambda light chains and heavy chains. The kappa gene consists of 250 V and 8 J segments. The lambda gene contains 200 V and 4 J segments. The gene for the heavy chain consists of 300 V, 8 J, and 4 D segments. If just somatic recombination and random combinations of light and heavy chains are taken into consideration, how many different types of antibodies can be produced by this species?This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?Sulfatase 1 (Sulf1) is a lizard blastema marker Identify a region of the Gekko japonicus Sulf1 protein sequence that is homologous with mouse Sulf1 (to educate antibody selection). hint: use NCBI Primer/Nucleotide Blast
- Using PEN and PAPER ONLY, illustrate a SARS-CoV-2 genome organization showing all the genes.Label the 4 structural genes and the 2 genes that code for the PP1a and PP1b and produces 16nonstructural proteins.Why is Hopkin's Cole test specific for tryptophan?The diagram in the first figure shows simplified versions of the immunoglobulin heavy chain locus, the T-cell receptor b chain locus, and the locus encoding the RAG-1 and RAG-2 recombinases. For the sake of this question, imagine that these diagrams represent all of the gene segments present in the immunoglobulin heavy chain and T-cell receptor b chain locus. You now analyze five individuals, each of which has a single inactivating mutation in a region of one of these three loci. These mutations are each indicated by a red ‘X’ in the second figure, and are numbered 1–5. For each of these inactivating mutations, indicate the alterations and/or defects that would be seen in the repertoire of antigen receptors found in mature B and T cells in that individual. Also, for each mutation, indicate whether the individual would likely show any immunodeficiency, such as a history of recurrent infections.
- An acute leukemia patient presents with blast morphology that is of neutrophil lineage with Auer rods and pseudo−Pelger-Huët nuclei, myeloperoxidase, and Sudan Black B. Cytogenetic analysis reveals a t(8;21)(q22;q22) translocation in the majority of the blasts. Which classification of AML best fits this morphology, immunophenotyping, and cytochemistry? Select one: a.AML with recurrent genetic abnormalities b.AML with multilineage dysplasia c.AML, therapy-related d.AML not otherwise categorizeduan used the ABO blood testing kit to determine his blood type. His test showed the following anti-A anti-B anti-D - - + + means agglutination was observed - means no agglutination was observed What is Juan's blood type? O- O+ A+ A-An example of dosage as it applies to blood group antigens is: Question 2 options: Every antibody specificity will react with cells with a homozygous expression Some antibody specificities may only react with an antigen with homozygous expression Some antibody specificities may only react with an antigen with homozygous expression Every antibody specificity will react with cells with a heterozygous expression