Which of the following BEST states genome editing in plants? A. transform large DNA constructs by suppression and activation B. insert a functional cis-regulatory element in the natural genes transgene vector C. assemble and synthesize large DNA molecules in a single transgene vector D. deletion, mutation, or integration of the gene of interest depending on the target trait
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Which of the following BEST states genome editing in plants?
A. transform large DNA constructs by suppression and activation
B. insert a functional cis-regulatory element in the natural genes transgene vector
C. assemble and synthesize large DNA molecules in a single transgene vector
D. deletion, mutation, or integration of the gene of interest depending on the target trait
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- A paper hypothesizes that white flowers are unable to produce anthocyanins (purple pigments) because they lack a functional “A” protein. However, it is also possible that an unknown gene is responsible for the lack of anthocyanins. Now that they have isolated DNA sequences of the “A” allele, design an experiment to use these DNA sequences to distinguish between these two hypotheses.After much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2. The gene associated with the mut1 mutation is called COB1 (i.e. mutations in COB1 cause the mut1 phenotype.) The researcher extracts DNA and RNA from developing ears of wild type, mut1 and mut2 plants. From the DNA samples COB1 PCR primers are used to amplify the coding region of the COB1 gene (see DNA gel below). An RNA gel is used to examine the expression of COB1 all three plants (see RNA gel below). results in photo: Which of the following is TRUE about the COB1 gene. a. There is a deletion in the COB1 gene in the mut1 plants. b. COB1 is not transcribed in the mut2 plant. c. There is a deletion in the COB1 gene in the mut2 plants. d. COB1 is not transcribed in the mut1 plant.What is mutation? Explain the significance of mutation in plant breeding. Give an example of a disease resistant variety of cultivated plant induced by mutation.
- Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools. Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (TO-34) containing one copy of crylla detected by Southern blot. Protein was expressed in high concentration at 45 davs after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A…Beadle and Tatum’s BEST contribution to how gene expression is understood can be summarized as what? A. One gene produces one protein. B. Independent assortment occurs. C. DNA is the genetic molecule. D. Information flows from DNA to RNA to protein.Which of the following statements about qPCR is true? a. The Ct value is generally directly proportional to the level of gene expression b.The intensity of fluorescence signal is generally inversely proportional to the level of gene expression c.The Ct value is generally inversely proportional to the level of gene expression d.The intensity of fluorescence signal is generally directly proportional to the level of gene expression
- Debate whether it is ethical for seed manufacturers to replace one controversial technique (genetic engineering) with another (mutagenesis) to avoid regulatory threats.a) How are mutations beneficial for plant breeding? Discuss with an example b) Explain in brief the technology that made us self-sufficient in food production.Briefly explain how Genome Wide Association Studies (GWAS) are done. Then, using this example of a Manhattan Plot explain what each of the X-axis and the Y-axis signifies in a GWAS.
- The genetic modification of plants for food is a hot-button issue around the world. Genes transplanted to corn, potato, soybean, and other food strains can confer desired traits, such as resistance to pests, which lessens the need for toxic pesticides. Though genetically modified foods are thought to be safe to eat, their impact on the environment may not be fully known. Do you worry about the long-term effects of genetically modified foods on your health, or the health of the environment? Do the positive impacts outweigh the negatives?Can Backcross breeding be used in crops? how? Cite your References. Subject - BiotechnologyGenetic engineering of plants provides an opportunity to alter their properties or performance in order to improve upon their utility. Such technology may be used to modify the expression of genes already present in the plants, or to introduce new genes of other species with which the plant cannot be bred conventionally. In fact, humans have engaged in genetic manipulation for millennia, producing plant and animal varieties through selective breeding and hybridization that significantly modify genomes of organisms. How has this technology created novel genetic information by specific alteration of cloned genes? (provide an example). Why do you think modern genetic engineering, has met with so much opposition? Should some forms of genetic engineering be of greater concern than others? Explain. In your response provide a specific example from literature to describe the implications of this technology (good or bad).