Which of the following is not a denaturing agent for proteins? Addition of heavy metals Addition of ethanol Addition of water Addition of strong acids
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- Heavy metal ions precipitate proteins from solutions in what way?The following are denaturing agents for proteins, except:* Please choose one correct answer only. A. Change in pH B. Heat C. Ultraviolet light D. Organic solvents E. None of the given optionsWhich of the following is an antioxidant? Vitamin E Glucose Cholesterol Palmitic acid Vicine
- Lead acetate solution is an agents of denaturation. Explain in 10 sentences the effect of each on proteins.What would be the best buffering agent to choose if you wanted to buffer an enzyme reaction or tissue culture medium at pH = 7.95? Group of answer choices a. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); pK = 7.5 b. Histidine; pK1= 1.78; pK2= 5.97; pK3= 8.97 c. Bicarbonate; pK = 6.1 d. Phosphate; pK1 =2.0, pK2 = 6.8; pK3 = 12.5 e. Tris or Tris(hydroxymethyl)aminomethane; pK = 8.07Which of the alternatives below indicates techniques used both to purify and to concentrate proteins, without losing the three-dimensional structure? a) diafiltration, affinity chromatography, centrifugation b) diafiltration, salting out, affinity chromatography c) precipitation with organic solvent, diafiltration, centrifugation d) centrifugation, precipitation with organic solvent, salting out
- The 3 proteins have the following properties. Which is the correct order of steps to extract them? + ethyl acetate, + (NH4)2SO4 at 20% saturation, centrifugation, + detergent (Triton X), buffer the lysate (pH=10), centrifugation, collect precipitate + ethyl acetate, + detergent (Triton X), + (NH4)2SO4 at 20% saturation, centrifugation, buffer the lysate (pH=10), centrifugation, collect precipitate + 1 M NaCl, + detergent (sodium dodecyI suIfate), centrifugation, +(NH4)2SO4 at 60% saturation, buffer at pH 7, centrifugation, collect precipitate + ethyl acetate, adjust pH to 7.3, centrifugation, collect supernate, +detergent (Triton X) to precipitate, +(NH4)2SO4 at 30% saturation, centrifugation, collect precipitateIn cation exchange chromatography, positively charged proteins exit the column first. In anion exchange chromatography, negatively charged proteins exit the column first. Group of answer choices The FIRST statement is TRUE. BOTH statements are TRUE. The SECOND statement is TRUE. NONE of the statements are TRUE.which of the following can be used as a disinfectant on a table top and works by denaturing bacteria lipids and membranes? 1-1% tincture of iodine 2-37% Formalin 3-Crystal Violet 4-Hand soap 5-Ethanol
- A cationic microbacteriaI peptide is anaIysed using cation exchange chromatography where the packing materiaI has a negative (-) charge. Choose which buffer system is best used. trishydroxyIammonium ammonia trisaminomethane phosphateAnaerobic bacteria produces which of the following inhibitory toxic product? -methilane -cyanide oxides -fatty acids -sulphidesWhich of the following amino acid can be particularly detected with test using NaOH and lead acetate as reagents? a.tyrosine b.tryptophan c.cysteine d.valine