Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1 ml 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample= number of bacteria/ml (For example, if 32 colonies are on a plate of ¹/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright ©2004 Pearson Education, Inc., publishing as Benjamin Cummings

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 23RQ
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Which of the following plates (dilution factor)
would you count/use to determine the original
bacterial count?
1 ml
Original
inoculum
Dilutions
Plating
1:10
9 ml broth
in each tube
1:10
1 ml
1 ml
1 ml
1:100
1 ml
1:100
2 (1:100)
3 (1:1000)
4 (1:10,000)
5 (1:100,000)
1:1000
1 ml
1 ml
1:1000
1:10,000
1 ml
1 ml
1:10,000
1:100,000
1 ml
1:100,000
Calculation: Number of colonies on platex reciprocal of dilution of sample=number of bacteria/ml
(For example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.)
Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
Transcribed Image Text:Which of the following plates (dilution factor) would you count/use to determine the original bacterial count? 1 ml Original inoculum Dilutions Plating 1:10 9 ml broth in each tube 1:10 1 ml 1 ml 1 ml 1:100 1 ml 1:100 2 (1:100) 3 (1:1000) 4 (1:10,000) 5 (1:100,000) 1:1000 1 ml 1 ml 1:1000 1:10,000 1 ml 1 ml 1:10,000 1:100,000 1 ml 1:100,000 Calculation: Number of colonies on platex reciprocal of dilution of sample=number of bacteria/ml (For example, if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 x 10,000 = 320,000/ml in sample.) Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings
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