Which of the following would restore a gene back to its proper reading frame? A. one insertion that contained 3 nucleotides B. three insertions that contained two nucleotides each C. one deletion of 2 nucleotides and one insertion of 3 nucleotides. D. two insertions that contained one nucleotide each

Which of the following would restore a gene back to its proper reading frame? A. one insertion that contained 3 nucleotides B. three insertions that contained two nucleotides each C. one deletion of 2 nucleotides and one insertion of 3 nucleotides. D. two insertions that contained one nucleotide each

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 7RQ: What is the most challenging issue facing genome sequencing? a. the inability to develop fast and...

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(a) Why can there be multiple codons for an amino acid? Why would this have evolved? (b) What is the advantage of Illumina Next Generation Sequencing?
Which of the following mutations would have the greatest negative impact on the protein product of a gene? A. a single base deletion close to the end of the coding region of a gene. B. a single base insertion near the start of the coding region of the gene C. a base-pair substitution D. a deletion of three bases near the middle of the gene
Describe What are the sticky ends of the restriction fragments? Select one: a. The surfaces of sticky ends contain matching base pairs, allowing fragments to splice. b. The surfaces of sticky ends have glue like substance that allow fragments to splice. c. The surfaces of sticky ends contain the exact same nucleotides, allowing fragments to bond. d. The surfaces of sticky ends have velcro like structure, allowing fragments to bond.
What is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the above
A contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.
The BLAST program begins with a particular genetic sequence anda. translates it into an amino acid sequence.b. determines if it contains one or more genes.c. identifies homologs within a database.d. does all of the above.
Base analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.
Tautomerization: A. Causes frameshift insertions B. Causes frameshift deletions C. Results in permanent chemical changes in nitrogenous bases D. Cause transition mutations E. Cause transversion mutations
Which type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertion
If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organism
Use the sequences below to determine what type of mutation has occurred by comparing the normal sequence to the mutated sequence. Normal Gene Sequence: 3'- ATAGCTAAGCCCATGCGG-5' Mutated Gene Sequence 3'-ATAGCTAAGCCCAGGTGCGG-5' A. Deletion - Point Mutation B. Insertion - Chromosomal Mutation C. Deletion - Frameshift Mutation D. Substitution - Point Mutation E. Insertion - Frameshift Mutation F. Insertion - Point Mutation
Restriction enzymes cut double-stranded DNA _____. a. at noncoding areas between genes b. between bacterial and viral DNAs c. at specific base sequences d. between purines and pyrimidines e. between promoter and operator DNA sequences