Suppose you want to make 500 mL of a 0.20 M Tris buffer at pH 8.0. On the shelf in your lab, you see a bottle of 1.0 M Tris at pH 6.8 and realize you can use this stock to make the new buffer. Assuming you have 5.0 M HCl and 5.0 M NaOH at your disposal, how much of each component would you need to add to make the 0.20 M Tris buffer at pH 8.0 from the 1.0 M Tris, pH 6.8 stock? (The pKa of Tris is 8.06.)
Suppose you want to make 500 mL of a 0.20 M Tris buffer at pH 8.0. On the shelf in your lab, you see a bottle of 1.0 M Tris at pH 6.8 and realize you can use this stock to make the new buffer. Assuming you have 5.0 M HCl and 5.0 M NaOH at your disposal, how much of each component would you need to add to make the 0.20 M Tris buffer at pH 8.0 from the 1.0 M Tris, pH 6.8 stock? (The pKa of Tris is 8.06.)
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter14: Equilibria In Acid-base Solutions
Section: Chapter Questions
Problem 25QAP: A sodium hydrogen carbonate-sodium carbonate buffer is to be prepared with a pH of 9.40. (a) What...
Related questions
Question
100%
Suppose you want to make 500 mL of a 0.20 M Tris buffer at pH 8.0. On the shelf in your lab, you see
a bottle of 1.0 M Tris at pH 6.8 and realize you can use this stock to make the new buffer. Assuming you
have 5.0 M HCl and 5.0 M NaOH at your disposal, how much of each component would you need to add
to make the 0.20 M Tris buffer at pH 8.0 from the 1.0 M Tris, pH 6.8 stock? (The pKa of Tris is 8.06.)
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 6 steps
Follow-up Questions
Read through expert solutions to related follow-up questions below.
Follow-up Question
Why didn't the tris stock of pH 6.8 play more of a role in the calculations? Should it of have?
Solution
by Bartleby ExpertKnowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Chemistry: Principles and Practice
Chemistry
ISBN:
9780534420123
Author:
Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:
Cengage Learning
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Chemistry: Principles and Practice
Chemistry
ISBN:
9780534420123
Author:
Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:
Cengage Learning
Chemistry: The Molecular Science
Chemistry
ISBN:
9781285199047
Author:
John W. Moore, Conrad L. Stanitski
Publisher:
Cengage Learning
General Chemistry - Standalone book (MindTap Cour…
Chemistry
ISBN:
9781305580343
Author:
Steven D. Gammon, Ebbing, Darrell Ebbing, Steven D., Darrell; Gammon, Darrell Ebbing; Steven D. Gammon, Darrell D.; Gammon, Ebbing; Steven D. Gammon; Darrell
Publisher:
Cengage Learning
Chemistry & Chemical Reactivity
Chemistry
ISBN:
9781337399074
Author:
John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:
Cengage Learning
Chemistry & Chemical Reactivity
Chemistry
ISBN:
9781133949640
Author:
John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:
Cengage Learning