Why do RNA viruses seem to have higher rates of mutation? Selected Answer: RNA nucleotides are less stable than DNA nucleotides. Answers: RNA viruses can incorporated a wide variety of non-standard nucleotides. Viruses are more sensitive to mutagens. Replication of their genomes does not involve proofreading. RNA nucleotides are less stable than DNA nucleotides.
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- Remdesivir is an antiviral drug used to treat SARS-CoV-2 infections. It is a broad-spectrum antiviral, meaning it has activity against many different viruses. Remdesivir can be incorporated into a new RNA chain as a virus replicates and can interfere with the viral replication machinery. Template RNA Elongation New RNA SARS-CoV-2 RNA polymerase Template groove Nucleotide entrance Nucleotides Remdesivir Figure 1. A representation of remdesivir and the viral replication machinery for SARS-CoV-2. 6. Figure 1 shows that remdesivir "mimics” an important component of RNA replication. Which specific component of RNA replication has a structure like that of remdesivir? 7. Propose a hypothesis about how remdesivir might inhibit the virus's replication process.Which viruses have potential for the highest mutation rates, DNA or RNA viruses? Explain O DNA viruses as DNA polymerases do not proofread thus make many mistakes/mutations when viral RNA is copied DNA viruses as DNA polymerases do not proofread thus make many mistakes/mutations when viral DNA is copied RNA viruses as DNA polymerases do not proofread thus make many mistakes/mutations when viral DNA is copied RNA viruses as RNA polymerases do not proofread thus make many mistakes/mutations when viral RNA is copied and DNA polymerases do proof readA" is a retrovirus. It can integrate DNA into random sites of human genome. Another virus "B" can integrate DNA at a specific site of human genome. Which one of these viruses would make a better vector for gene therapy? Briefly explain.
- The table below shows the properties of the genomes of three different viruses. The data were obtained as follows: Nuclease sensitivity was measured by the ability of deoxyribonuclease (DNase) or ribonuclease (RNase) to destroy the genome (a “+" means sensitivity). The ability of the genome to act as mRNA was tested by incubating it in a cell-free system. If amino acids were incorporated into protein, the data are shown as a Finally, the virus particles were tested for the presence of a virion polymerase. If an enzyme was present, the data show whether it could polymerize deoxynucleotide triphosphates (DNTPS) or nucleoside triphosphates (NTPS). "+. Genome Properties Nuclease Virion Can Genome Sensitivity? Polymerase? Be an mRNA? Virus DNase RNase With With DNTPS NTPS #1 - - #2 - - #3 For each virus, indicate the strategy of the genome, using the Baltimore classification. What is the nature of the product of the virion polymerase when present? + + + + + +Which of the following mutagens can substitute bases in DNA because of structural similarity? Group of answer choices nitrous acid aflatoxin base analogue intercalating agentWhich of the following viral nucleic acids can be used as a template to produce mRNA? Select all that apply. plus strand DNA plus strand RNA minus strand RNA minus strand DNA
- Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionWhat is the purpose of reverse transcriptase in retroviruses? It translates viral RNA into proteins. It converts host cell RNA into viral DNA. It uses viral RNA as a template for DNA synthesis. It uses viral RNA as a template for making more viral RNA strands.Which biological system contains a protein nucleocapsid surrounding 2 antiparallel polynucleotide strands (held together by hydrogen bonds), with deoxyribose sugars, but no ribose sugars? a single-stranded RNA viroid (like avocado sun blotch viroid) a double-stranded RNA virus (like the reovirus family) a single-stranded DNA virus (like fX174 virus of E. coli) a double-stranded DNA virus (like the smallpox virus) a single-stranded RNA virus (like tobacco mosaic virus)
- Some DNA vaccines use a brief and small electrical shock to get DNA in plasmids into cells. What advantage would there be in using DNA vaccines that consist of plasmids instead of just pieces of double-stranded DNA? The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the spike protein of the virus. The virus uses the spike protein to invade human cells where it replicates. Is it surprising that the mRNA must be stabilized with chemicals that need ultra-cold or frozen storage to protect the mRNA from degradation before it causes human muscle cells to make the spike protein? Why not just inject the double-stranded cDNA that codes for the spike protein of the virus? What additional step or steps would you need to use to get the human muscle cells to produce the spike protein if the cDNA was injected to serve as the virus?Viruses are made up of the same biomolecules as cellular organisms (DNA, RNA, proteins, lipids, etc.), which are synthesized through enzymatic dehydration reactions from building blocks and disassembled by enzymatic hydrolysis. They infect cells and replicate themselves using resources from the host cells. Scientists discovered a previously unknown strain of virus in the tropical Pacific Ocean and it can infect higher-order eukaryotes. Initial analysis revealed that the virus is mainly composed of nucleic acids, proteins, and lipids. Chemical analysis after complete hydrolysis revealed the following building blocks (by weight): Chemical components Nucleosides Amino acids Lipids, phosphates, and carbohydrates Percentage by weight 20 30 50 Analysis of percentages of nucleosides yielded the following results (by molar ratio): Nucleosides A G T C U Percentage by mole 19 29 19 29 4 Which of the following events mostly involves hydrolysis reaction after the virus infects…____________ proliferate by cutting and pasting the original copy to another part of the genome, while ____________ proliferate by reverse transcribing an mRNA copy of the original and inserting that into another part of the genome. Group of answer choices 1. Transposons; short tandem repeats 2. Retrotransposons; transposons 3. Retroviruses; transposable elements 4. Transposons; retrotransposons