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Why don't you heatfix a negative stain slide?
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- Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?Differentiate between the “hot method” and “cold method” of acid-fast staining.You mistakenly confused the primary stain and counter stain with crystal violet, how does you misture now appear when viewed with oil immersion?
- Is it possible to perform double or triple staining using fluorescent dyes? Explain.What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?