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Why is Endo Agar is recommended for membrane filtration experiment and not TSA plate?
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- Heat-labile material to be introduced to culture medium was filtered using membrane filters with unknown pore size. Effect: Rationale:Why is PBS buffer preferred when preparing a monolayer cell culture?How does gel filtration work? What are the applications of analytical versus preparative versions of the method ?
- You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solutionHow is it that smaller molecules move through a gel-filtration column more slowly than larger molecules, whereas in SDS polyacrylamide-gel electrophoresis (SDS-PAGE) the opposite is true: larger molecules move more slowly than small molecules?You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What should be your initial volume from the original culture? Show solution.
- You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is the dilution factor?Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?What concentration of agar is used in a semisolid medium for motility determination? How does that compare to a typical solid medium ? Explain.