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why is it important to apply the percent volume and percent retrieval in separation of mixtures experiment?
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- During melting point determination, what does melting point range means? How will you know if the sample is pure or not?Why do we use a makeup gas for some gas chromatography detectors?When you prepare your sample to run through the column chromatography , what is the first proper action you should do if you notice that your sample did not fully dissolve? A)Add a small amount of the sample B)Boil the sample mixture C)Use the rotovap D)Use a different solvent
- Chromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?Which of the following is NOT correctly related to the parts of paper chromatography set-up? a. Mobile phase: solvent b. Solute/Mixture: extract c. Stationary phase: paper d. Chromatographic chamber: sealed container
- Make a photocopy of the chromatogram given. Cut out the peaks and weigh them on an analytical balance. Use the weights to calculate the percentage of each component in the mixture.How does it affect the peak produced in gas chromatography when: – Inserting samples is too slow – Column temperature is too highWhy must a dry container be handled with tongs or finger pads when taking itsweight using analytical balance?
- A sample was placed on a chromatography column. Methylene chloride was used as the eluting solvent. No separation of the components in the sample was observed. What must have been happening during this experiment? How would you change the experiment to overcome this problem?In paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spotsPreparing a Thin Layer Chromatography experiment there are some common mistakes. Explain what happens in this situation: The solvent is above the line where the sample spots are