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- Why can transfer of DNA by conjugation cross genus lines, whereas transfer of DNA by natural transformation or phage transduction is usually limited to a few closely related organisms?When performing phage typing, how would one know whether that bacterium is killed by any of the bacteriophages?After sequencing E.coli ROAR340 strain and using Silico Clermont Phylotyper tools show that is it belongs to group B2 that has chuA, TspE4 and yjaA genes. Is ROAR340 pathogenic?