Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

Q: What information can be obtained from the slides prepared by wet mount and hanging drop technique…

A: A wet mount is produced by putting a fluid solution on a slide, suspending a specimen in it, and…

Q: Explain how to correctly use the microscope using oil immersion.

A: Light tends to bend while entering one material from a different one. The refraction of light…

Q: What is importance of labeling preserved specimen?

A: Labeling specimens: It is the process of naming an specimen so that it is easier to identified the…

Q: List the steps that you implement while using oil immersion to observe the specimen.

A: An instrument that helps to see very small objects is known as a microscope. There are different…

Q: The thickness of a specimen in view is known as the _______________.

A: Depth of focus.

Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…

A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…

Q: What situations would result in recollection or rejection of specimen? Determine  corresponding…

A: Specimen: a single animal, plant, mineral fragment, or other object used for scientific study or…

Q: What is the principle application of negative staining?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: What are the main characteristics of scanning electron microscopy?

A: Electrons are the negatively charged particles having minus 1 charge  and represented by e- ,…

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?

A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…

Q: What is the usage of the superresolution microscopy technique?

A: The microscope is identified as an optical instrument to observe very small objects. It is able to…

Q: What do you mean by deconvolution microscopy technique?

A: The organisms or substances that cannot be observed with unaided eyes are microscopic. The equipment…

Q: Calculate the size of an organism that occupies 26 um spaces under the scanner, LPO, and HPO using…

A: An ocular micrometer is used to measure the size of objects. The stage micrometer is used to…

Q: describe the agar-overlay technique

A: Biological techniques are processes or methods for trying to study living things. They include…

Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…

A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…

Q: Why should you always begin to use a microscope with a low-power objective?

A: Microscope is an instrument used to see the objects that cannot be seen by naked eye. A light…

Q: For which types of specimens is darkfield microscopy preferred over brightfield microscopy?

A: Microscope is an instrument used to view objects and organisms that are too small to be seen by…

Q: What alternative measures can be done if preservation of specimens is not possible?

A: The specimens collected has specific timeframes for these be fresh in nature. Only a fresh specimen…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: What is the significance of properly labeling collected specimen?

A: The specimen collected should be labelled properly before bringing it to the laboratory for testing.…

Q: What limitation applies to most forms of electron microscopy?

A: When we use Electron microscopy then we face certain limitations or drawbacks. Here we will discuss…

Q: If you prepared a sample of a specimen for light microscopy, stained it with the Gram stain, and…

A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…

Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…

A: Confocal microscopy uses light from laser, elimination of background information away from the focal…

Q: List four specimens that are better viewed through a dissecting scope than a compound light…

A: Hello! Since you have asked multiple question, we will solve the first question for you. If you want…

Q: a. b. What is the basic principle of image formation using this microscopy technique? What can be…

A: There are few important points : Scanning electron microscopy : It is very powerful and versatile…

Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?

A: Cell staining is a technique that is used to better visualize cells and cell components under a…

Q: Describe the process of bringing a specimen into focus using a compound light microscope.

A: A microscope is an instrument that can magnify specimen which is otherwise too small to be seen,…

Q: Light microscopy is used for which image? Image A Image B Image B, on the right O Image A, on the…

A: In the history of biological studies, various new tools and techniques have been developed so as to…

Q: laboratory? explain with diagram

A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…

Q: What are the key points necessary for proper collection ofclinical specimens, and why is it…

A: Clinical specimens are those biological specimens taken from a patient's body that will be used for…

Q: Which Two types of electron microscopy are in routine use in microbiology? Explain

A: The electron microscope is an instrument that uses the beam of electrons to examine objects on a…

Q: Explain in detail simple and differential staining and also improving and adjusting contrast in…

A: Staining is a chemical method which is used to colour the organism or the cell so that it is viewed…

Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…

A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…

Q: discuss the microscopy technique that gives the best contrast of nonbiological samples

A: Microscope is used those objects which are not visible under naked eye it is able to view the finer…

Q: How can colony morphology be used in the identification of bacterial species? What are the…

A: NOTE: AS PER OUR GUIDELINES, WE ARE ONLY SUPPOSED TO ANSWER THE FIRST THREE QUESTIONS, PLEASE…

Q: You are asked to calibrate the ocular micrometer for the 4x objective using the stage micrometer.…

A: Length of stage micrometer  is 1mm  with 100 divisions ,so each division is of size one-hundredth of…

Q: Explain why parfocal imaging is important, especially when viewing very small specimens using the…

A: Parfocal imaging involves the use of a parfocal lens that helps to keep the focus when the…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: Why are tissue specimen stained before viewing them using a micrscope?

A: If tissue and cells are observed under the microscope, then it would be very difficult to analyze…

Q: Why should freshly collected samples be used for microscopic examination?

A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…

Q: How is staining techniques useful in the clinical settings

A: Staining: A sample at the microscopic level can easily be visualized and distinguished from the…

Q: What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical…

A: Microscope is the widely used laboratory instrument in the molecular biology, it is mainly used to…

Q: Describe the use of microscopy to identify microorganisms in patient specimens

A: A microorganism, often known as a microbe, is a minute living entity. Microbiology is the study of…

Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…

A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…

Q: What is the purpose of examining your specimen slide using low power objective first nefore high…

A: Objective lens are magnifying lens that functions to magnify an image. These lens make an important…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.