Calculate the size of an organism that occupies 26 um spaces under the scanner, LPO, and HPO using stage micrometer and ocular micrometer if the calibration factor is 0.65, 0.75 and 0.85
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Calculate the size of an organism that occupies 26 um spaces under the scanner, LPO, and HPO using stage micrometer and ocular micrometer if the calibration factor is 0.65, 0.75 and 0.85
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- You are asked to calibrate the ocular micrometer for the 4x objective using the stage micrometer. You observe 32 stage micrometer units line up with 15 ocular micrometer units. What is the value for each division of the ocular micrometer for this objective? (Round to the 1 decimal place)Solve for the calibration factor in micrometers of the ocular micrometer using the LPOWhat factors must be addressed to properly acquire quantitative fluorescence microscopy images? What information about the imaging system should be recorded? What controls are needed and what standards are available?
- What are the differences between scanner, low power objective, high power objective and oil immersion objective? Introduce the terms stated above and explain comprehensively.If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?how do you purify a protein for single particle cryo-electron microscopy? please be specific and show steps.
- Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?compute for the callibration factor in micrometers of the ocular micrometer using the low power objective. explain your answerWhat information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
- Introduce and explain comprehensively the process of calculating the final magnification of specimens using a compound microscope. What is scanning objective and why it should be located at the beginning of the process?Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron micrograph (SEM)What is the difference between total magnification and objective magnification? You are looking at an organism on the 20x objective What is the total magnification? If the organism takes up 62 units on the ocular micrometer, how big is the organism is micrometers? Show your work.