Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: Enumerate the advantages derived from staining bacteria.
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: Please discuss why staining is important in visualizing microbes under the microscope. What…
A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…
Q: Explain why specimens should be taken aseptically and processed quickly to ensure accurate results,…
A: Microbiology is the study of microscopic organisms, such as bacteria, viruses, Achaea, fungi and…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: shape
A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: how can ultraviolet be used to control microbial growth. which specific UV can be used to control…
A: Ultraviolet (UV) - non-ionizing radiation. - penetration power is less. - It is a physical mutagen.
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: What are the advantages and disadvantages of observ-ing living-specimen slides and…
A: Microorganisms are the organisms that are not visible to the naked eye. Their size ranges in few…
Q: Outline the most signifi cant discoveries and events in microscopy, culture techniques, and other…
A: Microbes are minute, unicellular/multicellular, prokaryotic living organisms that cannot be seen…
Q: What color will a gram-negative cell be after Gram staining bythe conventional method?
A: Danish bacteriologist Hans Christian Gram developed the Gram staining method. This staining…
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…
A: Aseptic techniques means using practices and procedures to prevent contamination from pathogens. It…
Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…
A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…
Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…
A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…
Q: What advantages do confocal and deconvolution microscopy provide in comparison to conventional…
A: Confocal microscopy uses light from laser, elimination of background information away from the focal…
Q: Name four bacterial diseases for which the dark-fi eld microscope is an effective diagnostic tool.
A: Dark-field microscopy is a type of microscope that illuminates unstained samples. This technique…
Q: What are the advantages and disadvantages of using the Wet Mount technique?
A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…
Q: what is Microscopy and Staining of Microbes
A: Microbes are the tiny unicellular ,prokaryotics organisms having diverse varieties and habitats.…
Q: Please provide the principle of gram staining method.
A: Introduction: Gram staining is the most widely used differential staining. It is based on the…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: Explain in detail the study of microbial structure microscopy.
A: Answer: Microscopy : It is the technique which is used to enlarge the very small things so that we…
Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?
A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…
Q: A novice microbiology student incorrectly explains that immersion oil increases the magnification of…
A: The function of immersion oil is to increase the resolving the power of the light microscope. The…
Q: What major advantage does phase-contrast microscopy haveover staining?
A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…
A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…
Q: Evaluate the following preparations in terms of providing informationon microbial size, shape,…
A: Staining is an important step in observing the microbes under a microscope. they allow to the…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?…
A: In dark-field microscopy, microbes appear light in a dark background., viruses, macromolecules,…
Q: Distinguish the difference between a sterile media plate and a contaminated media plate.
A: streaking is a method to extract pure culture organisms from a different population of…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: How can colony morphology be used in the identification of bacterial species? What are the…
A: NOTE: AS PER OUR GUIDELINES, WE ARE ONLY SUPPOSED TO ANSWER THE FIRST THREE QUESTIONS, PLEASE…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: agnification is required to observe microorganisms?
A: A microorganism, often known as a microbe, is a microscopic organism that can be a single cell or a…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: what would be some strengths and weaknesses of using a Microscopic approach on microorganisms…
A: Answer: MICROSCOPIC APPROACH ON MICROORGANISMS: Microorganisms are the simple living organisms which…
Q: discuss the limitations in using the light microscope to study microbial communities.
A: Microscope is the most basic and useful instrument used in the microbiology laboratory. It is…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: Describe the use of microscopy to identify microorganisms in patient specimens
A: A microorganism, often known as a microbe, is a minute living entity. Microbiology is the study of…
Q: what are some of the strengths and weaknesses to using a Microscopic approach from a microbial…
A: Microscopic approach considers the behaviour of every molecule by using statistical methods. In…
Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?
A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…
What are the advantanges and disadvantages of using stained preparations for observing microbial cell morphology?
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- What are the disadvantages of doing a total microbial count by examining wet mounts under the microscope?Please discuss why staining is important in visualizing microbes under the microscope. What different types of staining methods can be used?What is the advantage of culture-independent analysis over culture-dependent analysis of microorganisms?
- Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?What advantage does the oil immersion objective have over the low power objective when doing bacterial examination?In preparing bacterial smear, why do we need to pass the slide over the flame? Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? Conclusion?
- Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?Briefly discuss the difference between simple and differential staining? Which is better suited for common clinical microbiology laboratory applications?What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?
- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.What are the advantages and disadvantages of observ-ing living-specimen slides and heat-fixed-specimen slides ofmicroorganisms?(a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside from bacteria, can gram staining be used with microbes such as fungi? Explain your answer.