Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid analysis method
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Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid analysis method
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- why are lysozyme and ovoalbumin the recommended standards used in the Bradford protein assay and why would they be preferred over BSA being this widely used but not recommended? Justify the answerWhy is the DNS method used in glucose assay?How would you determine the concentration of a protein that falls outside (above or below) the linear range of this assay? asap
- Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay experiment?What is the application of Bradford assay?There are three names that often get used when discussing the Lowry assay. Those names are: Lowry, Folin-Ciocalteu, and Biuret. Are those names interchangeable when discussing the assay or do those names describe the same thing(s)? Significant differences? What is the relationship between those names?
- What assay uses the fact that activators can be separated into DNA binding and activating domains? What information do you gain from this assay? Describe briefly how to perform this assay.Salmonella strains developed for the Ames test contain many special phenotypes that make them more useful for these techniques. Many of these phenotypes, including UvrA and rfa, make these strains mutate more easily, and therefore make the assays more sensitive. In 1-2 sentences each, state how the UvrA and rfa phenotypes make Ames test strains more sensitive to mutations.What is the difference between true, intragenic and a second-site reversion? Does the Ames test together with the s9 extract fall into any of these categories?