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does TGF-beta treatment cause cells to grow more or less in the soft-agar assay?
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- Will the results of an in vitro antibiotic sensitivity test always agree with the results of an in vivo use of the antibiotic? Why or why not?What is the difference between in-ovo and ex-ovo types of CAM Assay?What happens if you dont' add maltose in the molten top agar when performing an plaque assay? What would be the expected results?
- Why is it important in this assay to make sure that there is enough bacteria present to produce a lawn? What would happen if you had forgotten to add the E.coil to the soft agar? Why was the water bath set to 50 C? What is the difference between a CFU and a PFU?What is an ONPG test? WHat is he principle behind this assay.The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?