Why might adding a topoisomerase enzyme increase the sensitivity of an RT-Lamp assay. Would you expect the same effect if toposimerase was added to a RT-qPCR assay? Why or why not?

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
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Why might adding a topoisomerase enzyme increase the sensitivity of an RT-Lamp assay. Would you expect the same effect if toposimerase was added to a RT-qPCR assay? Why or why not?
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