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- 1. Describe the different types of analytes which can be analyzed using the different methods of optical analyses. 2. Why is it that absorbance readings be done at lambda max?1. In using the spectrophotometer PD 303, why is there a need to use water as blank when measuring the absorbance of each solutionAn ethanol solution of 3.5 mg/100 ml of compound Y (150.0 g/mol) in a 1.00 cm quartz cell has an absorbance (A) of 0.972 at λmax=235 nm. What is its molar extinction coefficient? Report your answer to the correct number of significant figures. Do not include units in your answer.
- An ethanol solution of 3.5 mg/100 ml of compound Y (150.0 g/mol) in a 1.00 cm quartz cell has an absorbance (A) of 0.972 at λmax=235 nm. What is its molar extinction coefficient? Report your answer to the correct number of significant figures. Do not include units in your answer. I need to know what is the correct number of sig figs for this question which will determine what the correct answer is.If triplicate absorbance readings of 0.419, 0.430, and 0.416 are recorded, what is the average %T for this solution? I've tried adding them all up and dividing by 3, adding only 2 of them up and dividing by 2, and even tried just entering one of the values and nothing has worked.Give at least two reasons for using very dilute solutions when doing fluorescence spectrometry
- 0.1120 g of a solid sample containing copper was dissolved by acid and transferred to a 250.00 mL volumetric flask and volume was made up with distilled water. The solution was then diluted by a factor of 25. The final solution was analyzed by atomic absorption spectrophotometry and the concentration was found be 2.982 ppm, What is the weight percentage of copper in the original solid sample? Keep four significant figures in your final answer.How will mistakenly using solution 1 to calibrate the spectrophotometer, instead of the blank effect the measured absorbance for solution 2 and the reported Kc? The blank contained 5.00mL of 0.00200 M Fe(NO3)3 (in 0.1M HNO3) and 5.00mL of 0.1 M HNO3. Solution 1 contained 5.00mL of Fe(NO3)3 (in 0.1M HNO3), 1.00mL of 0.00200 M NaSCN (in 0.1 M HNO3) and 4.00mL 0.1M HNO3. Solution 2 contained 5.00mL of Fe(NO3)3 (in 0.1M HNO3), 2.00mL 0.00200 M NaSCN (in 0.1 M HNO3) and 3.00mL of 0.1 M HNO3.You have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below If the standard solution (compound X) used have a concentration of 1 mM: 5. What is the reason for using test tube no. 1 in the table above? Can it be replaced withdistilled water?
- Which of the following does not impact the calculated concentration of a solution by absorption spectrophotometry? a. stray light generated during measurement b. absorptivity of the analyte c. height of the cuvette d. % Transmittance of the analyteWhat is the concentration of a sample that transmits 70% signal at a certain wavelength upon analysis? The molar absorptivity is 2.0 and the cuvette used has a width of 1.0 cm. Additionally, (a) What is the absorbance of the sample at this wavelength? b) And what is the fraction of light that would be transmitted by the sample if the concentration is increased three times?What is the design of figure II? FT IR interferometer single beam spectrophotometer diode array spectrophotometer double beam spectrophotometer