Why should the cell density of the inoculum be as small as possible? Why can we not standardize the inoculum against Mcfarland Standard #5 or even 10?

Q: If a slice of pancreatic tissue had been incubated in [ 3 H]leucine continuously for 2 hours, where…

A:

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Why do we need to look up values in the charts for each antibiotic?

A: Antibiotics have definitely transformed the lives of countless individuals by rescuing them from…

Q: Search for the principles and the uses of the following methods for protein measurements and provide…

A: In the question 5 different methods of protein measurement are given. Serum Protein Electrophoresis…

Q: Why is it not advisable to use adhesive mixture if protein histological  investigation are…

A: Immunohistochemistry (IHC) is defined as an important method helpful for pathologists in routine…

Q: Why would it be important for the Kirby-Bauer disc diffusion test to use a standard concentration of…

A: An antibiotic susceptibility test is the disk diffusion or agar diffusion test, or Kirby-Bauer test…

Q: What is the purpose of adding ice-cold ethanol to the cell lysate?   What is the purpose of…

A: the answer is with respect to the DNA isolation techniques:

Q: In size exclusion chromatography, which component will elute last in the set up?   Group of answer…

A: Size exclusion chromatography is also known as gel filtration chromatography (GFC) or gel permeation…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: What phrases do the CDC and other leading officials now use to describe the risk of contracting…

A: Covid-19 is a deadly, infectious disease that has resulted in a global pandemic. It is caused by a…

Q: What is the purpose of the TSI test?

A: A triple sugar iron test or TSI test is used to test a microorganism's ability to ferment sugar and…

Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…

A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…

Q: Total protein: Do you think that refractive index could be used as a regular method for serum…

A: Serum total protein determination is a biochemical test for measuring the total amount of protein in…

Q: What chemical structure should be present in an amino acid or protein to give a positive…

A: "Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: In patient with Maple Syrup Urine Disease, what specific amino acids should be not used in order to…

A: Maple syrup urine disease is an autosomal recessive metabolic disorder affecting branched-chain…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: Given that the normal range for serum protein is about 7 g/100ml and albumin is about 4 g/100ml,…

A: A total protein test measures the sum of all types of proteins in the blood. Proteins are…

Q: Why are platelets not considered cells? And why does it contain a kernel?

A: Platelets, or thrombocytes, are tiny, colorless cell particles in our blood that form clots and hold…

Q: What is the biological benefit(s) to the organism to have detachment induced cell death?

A: Anoikis is the programmed cell death that occurs in cells that detach from the surrounding…

Q: Why the synergy test results demonstrated that Penicillin G and Gentamicin did not act…

A: In the development of antibacterial drugs, when two or more drugs are tested together…

Q: What other method could be used to purify lysozyme from white using its unusual pl?

A: The unique charge properties of lysozyme with a very high pI of about 10.5 can be used in the…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: What are those methods being used in clinical practice of covid-19

A: Covid-19 is an acute respiratory disease caused by the infection of coronavirus. Some classical…

Q: The correct order of steps when purifying a protein are: Group of answer choices 1)Growing bacterial…

A: Recombinant/fusion proteins are made in expression model organisms. IPTG induce the expression.…

Q: Streptococci are divided into different groups called serotypes. Consult this or other textbooks and…

A: Streptococci are divided into many groups called serotypes based on the Lancefield classification…

Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?

A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…

Q: Enzyme chymotrypsin (enzyme digest) Samples: - 1 & 5- Standard Protein (Ladder) - 2 & 6- UNCUT…

A: Hypothesis: if similar bands are obtained for similar treatment then protein is structurally similar…

Q: what is the color of the capsule and color of the bacterial cell of streptococcus pneumoniae…

A: The capsule is a gelatinous layer that is secreted by certain bacteria surrounding their cell walls.

Q: Which of these methods will allow visualization of protein localization in a cell? a. Flow Cytometry…

A: Protein localization is the technique which allows the visualization of a protein inside the cell.…

Q: What are the conventional and unconventional substrates that are used in Single Cell Protein…

A: Edible unicellular microorganisms are referred to as single-cell proteins or microbial proteins.

Q: HIC chromatography does not yield 100% pure GFP. What other types of cellular proteins would most…

A: Hydrophobic interaction chromatography (HIC) HIC  is used to separate molecules on the basis of…

Q: In theory, if a 1-liter culture of rich medium is inoculated with 3 bacterial cells per milliliter,…

A: Generation time is the time taken for the doubling of the single cell. According to data given in…

Q: In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a…

A: The Kirby Bauer antibiotic test, also known as the disc-diffusion test, employs an antibiotic disc…

Q: If the D value of a microorganism is known, what else can we find? Explain by showing the…

A: D-value is the time required at a particular temperature to reach a log reduction that helps to…

Q: 2)Four proteins Cytochrome C (pI=10.2) Myoglobin (pI=7.2), Hemoglobin (pI = 6.8) and Serum Albumin…

A: In Electrophoresis pl is the isoelectric point. We generally know that negative ions attract to the…

Q: What are the raw materials and ideal properties of anti-aging cream

A: Aging is the process of becoming older. Cells are the basic unit of life. In higher eukaryotic…

Q: When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands…

A: Zymography is a technique to assess the enzymatic activity of proteins either in situ or by…

Q: If the inoculated lactose broth cube exhibits acid production but without gas formation, will you…

A: Phenotypic/bio-chemical characterization of microbes defines a set of bio-chemical tests used to…

Q: why is the recommended incubation procedure for the API 20e system 18 to 24 hours ?

A: Introduction The API 20 E is a non-fastidious or Enterobacteriaceae, and Gram-negative bacteria…

Q: Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most…

A: Growth media is the nutrients rich medium that helps in the growth of several bacteria and other…

Q: evidence of COVID-19 vaccination status considered Protected Health Information (PHI) under HIPAA…

A: PHI means protected health information which includes patients' health information such as a record…

Q: In making red blood cell suspension, why do we need to wash red blood cells in saline?

A: Red cell suspension is a common reagent used for many serologic procedures. Red cell suspensions…

Q: What volume of 2mg/mL BSA stock is needed to make 40uL of 100ug/mL BSA? Group of answer choices A)…

A: BSA refers to bovine serum albumin in an important constituent is molecular biology experiments.…

Q: Why is it not advisable to use adhesive mixtures if protein histological inevstigations are…

A: Histology is the study of microscopic anatomy (studies the anatomy of microscopic cells or tissues).…

Q: COVID 19 - cause respiratory distress and/ or intestinal distress?

A: The SARS-CoV-2 virus causes Coronavirus Illness (COVID-19), an infectious disease. The majority of…

Q: What are the identities and functions of the components of the Bradford reagent in protein content…

A: Different quantitative assays for the proteins are as follows: Biuret method Lowry's method…

Q: Monospot: inconclusive   Heterophile antibody titer of 1:56   Differential tests:…

A: Introduction The heterophile test is the most common and specific test to confirm the diagnosis of…

Q: After trypsinization of cells in the flask and cell counting, you determined a concentration of 10…

A: The detachment of cells from the adhering substrate is essential for specific cellular…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.