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What other method could be used to purify lysozyme from white using its unusual pl?
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- What is the result of this biochemical test in the gelatin deep?cWhich enzyme is this testing for? What tool is used to inoculate this medium?Explain how you would identify the separation of lysozyme from a mixed sample using electrophoresis.How can agarose gel electrophoresis process be imporved? What are ways to do it?
- given a phage lysate with 10^11 pfu/ml and a bacterial culture with 10^8 cfu/ml, how would you obtain MOI =0.1 ?When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reason for such an observation?why is Lysozyme treatment useful in bacterial cell lysis when protien isolation is happening.
- Is the telluride glycine agar? (TGA) a complex or defined medium? Explain based on its composition. What kind of Media is TGA explaining based on what kind of microorganisms it allows to grow?What is the benefit of using LB (Lysogeny broth) medium for the growth of E. coli, Bacillus subtilis and Pseudomonas putida? Why ?What is the effect of trypsin EDTA solution on monolayer cell culture preparation?
- What is the purpose of using Triton X in hemolytic assay.How might the technique of affinity chromatography be used to purify lectins ?in isolating ribosomes from a yield sample, describe the ideal type of centrifugation for this separation technique based on the following:*Type of Centrifugation* Type of fraction *Give 2 advantages of using this type of centrifuge.*Give 2 disadvantages of using this type of centrifuge.