Within a promoter, a transcriptional start site isa. located at the –35 sequence and is recognized by σ factor.b. located at the –35 sequence and is where the first base is used as atemplate for RNA transcription.c. located at the +1 site and is recognized by σ factor.d. located at the +1 site and is where the first base is used as a templatefor RNA transcription.

Within a promoter, a transcriptional start site isa. located at the –35 sequence and is recognized by σ factor.b. located at the –35 sequence and is where the first base is used as atemplate for RNA transcription.c. located at the +1 site and is recognized by σ factor.d. located at the +1 site and is where the first base is used as a templatefor RNA transcription.

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Description: Within a promoter, a transcriptional start site isa. located at the –35 sequence and is recognized by σ factor.b. located at the –35 sequence and is where the first base is used as atemplate for RNA transcription.c. located at the +1 site and is reco

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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a) what is a promoter and give the element and their functions of E.coli promoter b) what are eukaryotic transcription factor and list the class 2 general transcription factors and state their functions
f you made a change in the promoter sequence in the DNA that inactivates the promoter, what would happen at the RNA level? A-Nothing, because the RNA would be made as usual B-Transcription factors would be unable to bind and the RNA polymerase would not be recruited to the DNA, so no RNA would be made. C-The mutation of the DNA would be carried through to the RNA sequence. D-The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made. EXPLAIN WHY THE ANSWER YOU CHOOSE IS CORRECT
Specific transcription factors in eukaryotes interact with enhancers, which may be a long distance from the promoter. These transcription factors then a. alter the structure of the DNA between enhancer and promoter. b. do not interact with the transcription apparatus. c. can interact with the transcription apparatus via DNA looping. d. can interact with the transcription apparatus by removing the intervening DNA.
Which sequence is most likely to be found in a promoter? a) CGGTGTATATCGTAC b) GTACAGTCATCCCGT c) AAATCTACTACGATT d) GGGTTGGGTTGGGTT
a. Use circles for activators and squares for repressors. Draw the shapes bound to each promoter region. b. Under the word gene write “ON” or “OFF” to indicate if the gene is transcribed or not under the different conditions.
The “domain-swapping” experiment that grafts the Gal4 DNA-binding domain to the LexA activation domain generates a chimeric protein that will: A) bind to the Gal4 site. B) bindto the LexA site. C) activate transcription of the LexA gene. D) activate transcription of alleukaryotic genes. E) All of the answer options are correct.
Your investors are concerned that the GasP protein might not be sufficiently produced under normal laboratory conditions. They suggest controlling the transcription of the gasP gene using a chemical that will “trigger” its transcription. a. What type of promoter could be used? b. What chemical will you use to control transcription? c. How does this method of control work?
The promoter consensus sequence at the -10 BOX in the bio gene in E. coli was TAGACT; however, a transversion occurred which changed the G nucleotide to T, what outcome(s) are likely? a. transcription levels would decrease because the promoter would be weaker b. nothing would happen because the promoter would not change the mRNA sequence c. the mutation would block RNA polymerase from binding to the DNA d. transcription levels would increase because the promoter would be stronger e. the bio gene does not require a functional promoter
A scientist compares the promoter regions of two genes. Gene A’s core promoter plus proximal promoter elements encompasses 70bp. Gene B’s core promoter plus proximal promoter elements encompasses 250bp. Which of the scientist’s hypotheses is most likely to be correct? a. More transcripts will be made from Gene B. b. Transcription of Gene A involves fewer transcription factors. c. Enhancers control Gene B’s transcription. d. Transcription of Gene A is more controlled than transcription of Gene B.
What is one function of TFIIH during transcription? a. Recruiting the TATA-box binding protein to the TATA-box. b. Terminating transcription via factor-dependent termination. c. Capping the 5' end of mRNA with 7-methylguanylate. d. Phosphorylating the CTD of RNA polymerase.
Which of the following is true regarding General transcription factors? a. Act at every gene for a given RNA pol b. Act only at specific genes for under specific conditions c. Must be very close to the promoter d. None of the above
The effect that cAMP has on CAP is that a. it causes LacI to bind a promoter. b. it causes CAP to stabilize RNA polymerase binding of a promoter. c. it causes TrpR to bind a promoter. d. it causes RNA polymerase to not bind a promoter.