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- In this experiment, you preformed an extraction to separate the aqueous and organic solvent layers. Why was the organic solvent layer on the bottom in the separatory funnel? What would cause the organic solvent layer to be the top layer? Explain your answer.This experiment is the Separation of a mixture of 3-nitroaniline, Benzoic acid, and naphthalene using an acid-base separation extraction technique What properties of the three compounds in this experiment allow you to use the above technique to separate them?Why are you asked to use multiple portions of the solvent at the extraction steps?Why are you asked not to discard any solvents until you have obtained your products?In this lab you used acid/base extraction to remove acidic impurities, how could basic impurities be removed through extraction? Imagine the two compounds below are both dissolved in ethyl acetate (a standard organic solvent for extractions), create a diagram/flow chart that outlines steps that could be taken to separate them through acid/base extraction and recover each compound in its neutral Be sure to track when each compound is in the organic or aqueous layer.
- What is the reason behind the portion-by-portion evaporation of DCM in the extraction of eugenol?Why is methanol chosen as the solvent for recrystallization? What would happen if the student uses dichloromethane instead?What are emulsions? Why do they form during extractions? How is their formation minimized?
- TLC, a powerful analytical tool, can be used to monitor the progress of reactions. The synthesis of ethyl-3-coumarincarboxylate can be monitored by TLC by displaying the starting aldehyde 1, and coumarin product 2, which have very distinct Rf values (Hint: Think about the polarity of compounds 1 and 2 in terms of their abilities to H-bonds to silica gel). What can be determine about the progress of the reaction from analysis of the TLC shown below?If 80% of a desired compound is extracted into the organic phase in each step (leaving 20% in the aqueous layer), how many extractions are necessary to ensure that at least 99% of the compound has been recovered in the organic phase? 1 extraction 2 extractions 3 extractions 4 extractionsWhy is it required to add drying agent like sodium sulphate to the organic layer which may contain only a neutral compound? Explain
- Using the table on slide 7, what ORGANIC solvent could you use to do an extraction of something fromisooctane? What about from acetic acid? b. On slide 7, it says the solvents in grey would not work to extract an aqueous layer. Why not? 6. a. So if you were trying to extract 15mL of an aqueous layer, why wouldn’t you want to use 45mL of theorganic solvent? Why would 20mL be better? b. Why wouldn’t you use 2mL of the organic solvent?Q1: Give one lysis buffer that is commonly used for western blotting experiments and include its components Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Q4. Why is it necessary to store the prepared lysates in a very low temperature?What is the function of the sulfuric acid in the dehydration of alcohols to form alkenes? Why was it important to keep the Gas chromatograph close to room temperature during the analysis of the alkene mixture?