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- 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL of SOC medium, 100 μL of this solution was plated onto a TSA + ampicillin plate. Can you please show me how I calculate the total number of successful transformants per mL of competent cells plated. The total number of colnies counted on plate was 70. Thank youLooking at the model data i upload and given the question how could i analyse tboth experimental results (antibiotic selection and agarose gel). Including one clearly labelled image of the agarose gel and with an accompanying legend. And how could i interpret the findings of the results and draw a conclusion i.e. what is in each tube? Also is there any further experiments that I could perform to confirm the identity of the plasmid stocks?Since higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?
- Why are the following reagents used? Neutralizing solution (Plasmid isolation) Isopropanol (Plasmid isolation) RNase (isolation of genomic DNA)Which plasmid contains the therapeutic gene? 1 - the plasmids with SERCA2a 2- the viral plasmid 3- the helper plasmidWhat is the role of the following in the alkaline plasmid screen? G buffer (Cell Suspension Solution) Denaturing Solution (Cell Lysis Solution) Neutralization Solution
- Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.True or false White colonies in apicillin-agar plates using plasmids with lacZ gene as vector indicated both successful transformation and insertionhelp me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circular
- During the isolation of TOL plasmid from Pseudomonas putida, it was observed that the concentration of the plasmid DNA per 50 mL of sample was extremely low. Describe a technique you could employ to increase the concentration of the TOL plasmid.We transformed E coli cells with a plasmid modified to contain a 'virulence factor' which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp) Assume you were given competent cells of known transformation efficiency (TE). Assume TE= 1×10[6] (note 10[6] means 10 to the power of 6). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transtormation reaction? О 1.5.00 O 2.0.05 О 3.50.00 О 4.200.00 О 5.20.00 О 6.0.5016. Bacterial cell wall membrane includes all EXCEPT ( Mark both answers ) Group of answer choices O- polysaccharides porins lipoproteins asymmetric phospholipids membrane lipid A teichoic acids 18. F plasmid is best desribed as a ……………... plasmid Group of answer choices non self transmissible self transmissible virulence plasmid becos it codes for toxin promiscuous high copy number 19. Identify the FALSE statement from the following, Group of answer choices self transmissible plasmids have origin of transfer as well as mobilisation genes inclusions are non functional, storage granules accumulated inside bacterial cells plasmid DNA are in nucleoid area in Bacillus anthracis R plasmid codes for the sex pili host range refers to ability of plasmid to multiply in cell types 21. Which one of the following statement regarding plasmid is FALSE, Group of answer choices they are small, extrachromosomal DNA molecules they are necessary for…