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Draw a graph based on the result. And give a short discussion

Draw a graph based on the result. And give a short discussion

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Draw a graph based on the result. And give a short discussion based on group1, group 2 and group 3.

Procedure A Add ul ampicillin to Take 3 ml 50 of Top up to 50ml. to Add fresh LB- Mix Sml the mixture overnight culture- with 45 ml LB mixture analyse OD600 the both broth The mixture is then Withdraw 3ml mixture to Overnight culture Measure every 15 mins. Stop until analyse (OD600)record as zero time-point. incubated shaked incubator at 37°c 200rpm at (E-coli + LB borth) 75 mins. Sml Mix overnight culture B (control) with 45 ml LB Take 3 ml Add 50 ul ampicillin to of mixture to No ampicilin the analyse OD600 the mixture broth Withdraw 3ml mixture to - analyse (OD600)record as zero time-point. The mixture is then Measure every 15 mins. Stop until 75 mins. - incubated at shaked incubator at 37°c 200rpm Result Group 1 Group 2 Group 3 (A) (A) OD 600 Time (minutes) (B) (A) (B) (B) Ampicilin 0.315 Ampicilin Control Control Ampicilin Control 0.124 0.248 0.289 0.133 0.121 15 0.756 0.367 0.485 0.162 0.168 0.167 30 0.645 0.428 0.757 0.057 0.248 0.053 45 0.633 0.695 1.025 0.052 0.339 0.018 60 0.599 0.937 1.434 0.05 0.509 0.053 75 0.593 1.301 1.778 0.039 0.676 0.053
Transcribed Image Text:Procedure A Add ul ampicillin to Take 3 ml 50 of Top up to 50ml. to Add fresh LB- Mix Sml the mixture overnight culture- with 45 ml LB mixture analyse OD600 the both broth The mixture is then Withdraw 3ml mixture to Overnight culture Measure every 15 mins. Stop until analyse (OD600)record as zero time-point. incubated shaked incubator at 37°c 200rpm at (E-coli + LB borth) 75 mins. Sml Mix overnight culture B (control) with 45 ml LB Take 3 ml Add 50 ul ampicillin to of mixture to No ampicilin the analyse OD600 the mixture broth Withdraw 3ml mixture to - analyse (OD600)record as zero time-point. The mixture is then Measure every 15 mins. Stop until 75 mins. - incubated at shaked incubator at 37°c 200rpm Result Group 1 Group 2 Group 3 (A) (A) OD 600 Time (minutes) (B) (A) (B) (B) Ampicilin 0.315 Ampicilin Control Control Ampicilin Control 0.124 0.248 0.289 0.133 0.121 15 0.756 0.367 0.485 0.162 0.168 0.167 30 0.645 0.428 0.757 0.057 0.248 0.053 45 0.633 0.695 1.025 0.052 0.339 0.018 60 0.599 0.937 1.434 0.05 0.509 0.053 75 0.593 1.301 1.778 0.039 0.676 0.053
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