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A: The AluQuant system used for human deoxyribonucleic acid quantitation uses certain probes that help…
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A: DNA fingerprinting: - It is a method which provide genetic information of any living thing. It…
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A: DNA extraction is the procedure of isolating the Deoxyribonucleic acid from biological samples. PCR…
Q: What are STRs, and why are they useful for DNA profiling?
A: Introduction In the genome there are usually two type of nucleic acid sequence present. One is Non…
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A: NGS is an advanced DNA sequencing technique used high throughput and massively parallel sequencing…
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A: Deoxyribonucleic acid or DNA is a polymer composed of two polynucleotide chains that coil around…
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A: DNA is extracted from specific cells to study the extracted DNA. The purity of extracted DNA is…
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A: Ribosome profiling- it is carried out on a split sample, with parallel libraries constructed for…
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A: DNA polymerase is an enzyme. A primer is a single-stranded DNA fragment that binds to template DNA…
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A: Codis is a database used by fbi team to find out the criminal from their stored database. This…
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A: DNA microarray is basically a collection of microscopic DNA spots that are attached to the solid…
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A: The probe is basically a single stranded sequence of DNA or RNA which is used commonly for the…
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A: DNA sequencing is basically the process by which the nucleic acid sequence can be determined. the…
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A: Gel electrophoresis is a method used to segregate the DNA fragments depending on their size and…
Q: t tandem repeats) multiplexing combined with PCR requires a relatively large volume of high quality…
A: Short tandem repeats are a small sequence of nucleotides containing repetitive nucleotides ranging…
Q: Sanger sequencing Write a precise and accurate differential report on the above sequencing…
A: DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a…
Q: Discuss the use of gel electrophoresis for the separation of macromolecules (DNA, RNA and protein)…
A: Gel Electrophoresis It is a technique which is used to separate the fragment of macromolecules like…
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A: Deoxyribonucleic acid or DNA is the hereditary material that is present in all organisms. DNA…
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A: PCR - (polymerase chain reaction) is used to amplify the DNA sequence through repetitive cycles. It…
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A: DNA is genetic material in almost all living organism that transit from one generation to another
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A: Because plasmid is a circular DNA molecule, this is the case. It becomes linear but not fragmented…
Q: Which best desrcibes a 'CRISPR'? a delivery vector a small DNA binding protein a…
A: The correct option is - clusters of short palindromic DNA .
Q: what is the Jusage of DNA separation in gel electrophoresis
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Q: A DNA sample was sent off for Sanger sequencing. The results are shown below. What is the sequence…
A: The Sanger sequencing is also known as the chain termination method, it is a method used to…
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A: Introduction: Probes are the small DNA or RNA strands that are used to label DNA. The labeling of…
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A: The tiny living creatures such as bacteria, viruses, fungus, algae, and protozoa have a significant…
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A: In biotechnology procedures, a ___ is a nucleic acid fragment that is used to search for and…
Q: Recombinant DNA techniques that require gel electrophoresis
A: Following are the applications of gel electrophoresis in DNA recombinant technology.
Q: Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.
A: Main components for preparation of cloning vector are : Origin of replication Marker gene…
Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…
A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…
Q: Microarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although…
A: A usual microarray technology includes the hybridization of an mRNA with its original template of…
Q: An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short…
A: Introduction In the genome, there is usually two types of nucleic acid sequence present. One is…
Q: The blotting technique used toidentify the isolated protein is ________A. Northern blottingB.…
A: Proteins are the type of macromolecules that are synthesized in the cytoplasm during the process of…
Q: Ruwaifa want to compares a nucleotide query sequence what option he will opt in BLAST and why?
A: BLAST is Basic local alignment search tool used to compare the queries in a DNA database. They are…
Q: write the pros and cons of crispr technology in bullet points with explaintion
A: Crispr technology is a technology that edit genes. It is process of finding specific DNA inside the…
Q: Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why…
A: The Prokaryotes have a 70S type of ribosome (S=Svedberg units). The 70S ribosome is made up of a 50S…
Q: The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis…
A: PCR is a process in which millions of copies of a specific sequence of DNA can be made in a matter…
Q: Aside from gel electrophoresis Give another method to quantify DNA. Explain the concept behind this…
A: DNA quantification is a type of quantification of nucleic acids that is used for the determination…
Q: In assessing extracted DNA quality, what does A230 indicate? nucleic acids background reading…
A: DNA and RNA were characterized using several methods for assessing quantity, quality, and molecular…
Q: Short tandem repeats (STRS) in DNA are often used in forensic analysis and for determining…
A: Short tandem repeats (STRs) are short repeated successions of DNA (2-6 bp) that record for roughly…
Q: give the significance/role/effect of the reagent/condition in the isolation or analysis of a…
A: DNA isolation is a process of isolation of DNA from biological sample like body fluid, tissue, etc.…
Q: Describe several applications of DNA profiling and microarrayanalysis.
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: To achieve a 10x depth coverage for a genome of length 10 Mbp, how many reads of 100 bp are…
A: A Coverage is multiplier based on total size of the genome. Formula = Coverage = (read length) x…
Q: Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two…
A: Recombinant DNA technology comprises altering genetic material outside an organism to obtain…
Q: Index sequences that are small segments of DNA with sequences that are unique and not found anywhere…
A: The DNA is the genetic material in most of the organisms. it is located within the nucleus of the…
Q: Describe two different types of protein microarrays, and discuss their uses.
A: Genomics and proteomics are relatively new branches of genetics. Genomics is the study of all the…
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- Question:- 1. Brassica oleracea is the wild plant from which brussels sprouts, cabbage, broccoli, and cauliflower were domesticated. Why is it important to keep many diverse seeds from the forb B. oleracea across its range? A. If our B. oleracea-derived crops are driven to extinction, we can re-domesticate them from the wild plant. B. Our crop Brassicas are much more genetically diverse than B. oleracea because of extensive breeding programs, so we need to conserve as much of B. oleracea's diversity as possible. C. The budding pattern in Brassica may be further modified to produce more productive crop plants. D. B. oleracea may have gene variants that can be incorporated into our domesticated plants to increase their environmental range or tolerance to pests. E. We may be able to domesticate another crop plant from B. oleracea.Question- 1. In onions, male sterility is due to the interaction of a chromosomal allele pair hh and “sterile” (S) cytoplasm. All other combinations (i.e. HH/Hh and “sterile” cytoplasm, HH/Hh or hh “fertile” (F) cytoplasm) result in male-fertile plants. The male-sterile trait is incorporated into inbred lines to produce hybrid F1 seed on a commercial scale.a) How would you perpetuate the male-sterile line? Show the cross.b) Briefly outline the method of producing hybrid seed (heterozygote) for the commercial crop. Show the cross.c) Does it matter whether the cytoplasm is fertile or sterile in the male-fertile inbred? Explain.QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology. QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes.
- QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology.QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes. QUESTION NO. 3…Question 23 Heterochromatin is the part of the DNA where genes are transcribed actively Question 23 options: True False Question 24 Chromatin Immunoprecipitation is used to : Question 24 options: Isolate DNA binding proteins Identify specific sequences bound to Transcription factors Identify a DNA binding peptide Identify high throughput sequenceQUESTION NO. 1 A transition mutation A. occurs when a purine is substituted for a pyrimidine or vice versa. B. results from the insertion of one or two bases into the DNA chain. C. is most frequently caused by chemicals (like acridine) that intercalate into DNA. D. results from substitution of one purine for another or of one pyrimidine for another. E. always is a missense mutation QUESTION NO. 2 Degeneracy of the generic code denotes the existence of A. multiple codons for a single amino acid. B. codons consisting of only two bases. C. base triplets that do not code for any amino acid. D. different systems in which a given trip let codes for different amino acids. E. codons that include one or more of the unusual bases. QUESTION NO. 3 Replication A. requires that a phosphodiester bond of the incoming dNTP be hydrolyzed in order to be added to the growing chain. B. uses 5' to 3' polymerase activity to synthesize one…
- Question:- 1. A brand-new deleterious allele has just entered a population via mutation; this allele had not existed before in the population. If its allele frequency in the next generation is 0.00000021, what must have the mutation rate have been of these gene? 2. Use #1 to answer. Using standard scientific notation format, enter the exponent (including the negative sign).Question 16: One of the two genes known to be mutated in cases of Hypokalemic periodic paralysis (which is inherited in an autosomal dominant pattern but known to affect males more often than females) is the calcium voltage-gated channel subunit alpha1 S (CACNA1S). What is known about the gene is recorded here: https://www.ensembl.org/Homo_sapiens/Gene/Summary?db=core;g=ENSG00000081248;r=1:201039512-201112451 Please navigate to the link above and use the information and link-outs from the page to answer the following question. GIVE YOUR ANSWER AS A NUMBER ONLY, NO UNITS: What is the size in amino acid residues of the CACNA1S transcript named CACNA1S-202?Question People who carry a theoretical genetic disorder (called B-disease) can be identified from a 2kb DNA sequence. People who carry this genetic disorder have a single nucleotide polymorphism that results in a change of GTATTC to GGATTC, a site that only occurs once at nucleotide number 750 in this DNA sequence. Answer the following questions based on the information provided. (a) How can you develop a simple molecular test to identify the genetic disorder? (b) If you have carried out the molecular test (based on the information above) on a 100 individual and found that 24 were healthy (BB) and 26 were carriers (bb); (1) What is the ratio of heterozygous? (2) Show how can you identify the three types from the agarose gel.
- Question 12 One of the two genes known to be mutated in cases of Hypokalemic periodic paralysis (which is inherited in an autosomal dominant pattern but known to affect males more often than females) is the calcium voltage-gated channel subunit alpha1 S (CACNA1S). What is known about the gene is recorded here: https://www.ensembl.org/Homo_sapiens/Gene/Summary?db=core;g=ENSG00000081248;r=1:201039512-201112451 Please navigate to the link above and ensure that you click to reveal the transcript table. Then use the information in the table to answer the following question. PLEASE GIVE YOUR ANSWER AS A NUMBER ONLY, NO UNITS What is the size in base pairs of the CACNA1S transcript named CACNA1S-202? Answer: The size of the CACNA1S transcript named CACNA1S-202 is Blank ... base pairs.Question 14 One of the two genes known to be mutated in cases of Hypokalemic periodic paralysis (which is inherited in an autosomal dominant pattern but known to affect males more often than females) is the calcium voltage-gated channel subunit alpha1 S (CACNA1S). What is known about the gene is recorded here: https://www.ensembl.org/Homo_sapiens/Gene/Summary?db=core;g=ENSG00000081248;r=1:201039512-201112451 Please navigate to the link above. Use the left-hand menu to view the sequence for CACNA1S, and answer the following question. ANSWER ONLY IN UPPERCASE LETTERS, NO UNITS. What are the last six nucleic acid bases of exon 1? Answer: The last six nucleic acid bases of CACNA1S, exon 1 are Blank ...Question 13 One of the two genes known to be mutated in cases of Hypokalemic periodic paralysis (which is inherited in an autosomal dominant pattern but known to affect males more often than females) is the calcium voltage-gated channel subunit alpha1 S (CACNA1S). What is known about the gene is recorded here: https://www.ensembl.org/Homo_sapiens/Gene/Summary?db=core;g=ENSG00000081248;r=1:201039512-201112451 Please navigate to the link above and use the information and link-outs from the page to answer the following question. GIVE YOUR ANSWER AS A NUMBER ONLY, NO UNITS: What is the size in amino acid residues of the CACNA1S transcript named CACNA1S-202? Answer: The size of the CACNA1S transcript named CACNA1S-202 is Blank ... amino acid residues.