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- Solution. Vol. of A solution (mL) [methyl red], M Absorbance 1 10.00 _1.64 x10^-16_ _0.256_ 2 15.00 _2.45 x10^-16_. _0.373_ 3 20.00 _3.27 x10^-16_. _0.486_ 4 25.00 _4.09 x10^-16_. _0.620_ Plot a graph of the absorbance (y axis) as a function of the concentration of methyl red (x axis). What is the slope of the graph?A standard curve of absorbance vs. concentration has a linear trendline of y = 0.56x + 0.987. Use this trendline to calculate the concentration of an unknown sample that has an absorbance of 1.245.2. ] You make 150.0 mL of a copper(II) sulfate solution but forget to cover it before leaving for the day. The next class, you measure the volume and find that you only have 132.0 mL remaining. You didn’t record the initial concentration, but you measure the absorbance of the remaining solution at 620 nm and find that it is 0.386. You also construct the following calibration curve for CuSO4: Standard solution Abs620 0.50 M 0.424 0.40 M 0.336 0.30 M 0.247 0.20 M 0.159 (refer to image) a. What is the value for k (in Beer’s Law)? Provide a calculation or graph to support your answer. b. What is the concentration of the (remaining) solution? c. What was the concentration of the original solution from the first class? d. If you removed 7.5 mL from the 132.0 mL solution in the process of measuring the absorbance, how much water should you add to obtain the original concentration that you solved for in part (c)?
- A student prepared four standard solutions of (NH4)2Fe(SO4)2 and measured their absorbance at 505 nm. She created a calibration plot with the best-fit line of y = 9372 x + 0.0539. She measured the absorbance of her diluted unknown sample solution as (4.69x10^-1) nm. If she prepared her diluted sample solution by diluting 10.00 mL of the original solution to a total volume of 50.00 mL with deionized water, what is the concentration of Fe2+ in her original solution? Answer in units of M. Note: Your answer is assumed to be reduced to the highest power possible.You are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or A595) of your unknown is greater than that of the highest-concentration standard solution. It is not advisable to simply extrapolate the line of the standard curve to calculate the concentration of the unknown In this scenario, what would be the best strategy to measure the concentration of your unknown (instead of extrapolating from the standard curve)? (answer in 5 sentences)What absorbance corresponds to 99% transmittance? To 0.10% transmittance?
- A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. To get the concentration of amine in this compound, you subject it to spectroscopic analysis. So, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample then you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. Solve for the molecular weight of the compound. The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound?A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound? I need help with part B of this question.
- A standard curve was performed using an Absorbance spectrophotometer, and the equation of that line was determined to be y = .1563x + .025. The absorbance of an unknown was measured at .35. Calculate the concentration of the unknown according to the standard curve.Five standard chromium (VI) solutions were prepared through serial dilution technique with corresponding absorbance readings at 540 nm wavelength (as follows: Absorbance Concentration A1 = 0.650 C1 = 1.9 ppm A2 = 0.712 C2 = 4.0 ppm A3 = 1.370 C3 = 6.0 ppm A4 = 2.050 C4 = 8.0 ppm A5 = 3.423 C5 = 10.0 ppm The sample solution gives an average absorbance reading of 0.713. What is then the concentration of chromium (VI) ion in the sample?Five standard chromium (VI) solutions were prepared through serial dilution technique with corresponding absorbance readings at 540 nm wavelength as follows: Absorbance Concentration A1 = 0.650 C1 = 1.9 ppm A2 = 0.712 C2 = 4.0 ppm A3 = 1.370 C3 = 6.0 ppm A4 = 2.050 C4 = 8.0 ppm A5 = 3.423 C5 = 10.0 ppm The sample solution gives an average absorbance reading of 0.713. What is then the concentration of chromium (VI) ion in the sample? (Note: please provide necessary solutions)