You are running a 500L Protein A purification column to purify a 150,000 Da monoclonal antibody. Based on data from previous experiments, you know that the association constant between Protein A and this monoclonal antibody is 64 and that the dynamic binding capacity of the column is approximately 0.33 m.M. Historical experience with the column indicates that the elution step is only 85% efficient. If, after loading the column, you measure a concentration of monoclonal antibody in the mobile phase to be 0.02, how much monoclonal antibody (in kg) should you expect to M elute from the column?

You are running a 500L Protein A purification column to purify a 150,000 Da monoclonal antibody. Based on data from previous experiments, you know that the association constant between Protein A and this monoclonal antibody is 64 and that the dynamic binding capacity of the column is approximately 0.33 m.M. Historical experience with the column indicates that the elution step is only 85% efficient. If, after loading the column, you measure a concentration of monoclonal antibody in the mobile phase to be 0.02, how much monoclonal antibody (in kg) should you expect to M elute from the column?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 9QP: a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein...

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