You are studying the kinetics of a novel competitive inhibitor of ATP-citrate lyase that can be used to prevent obesity. What is likely true about this inhibitor? (A) This inhibitor lowers the Vmax of ATP-citrate lyase. (B) The inhibitor likely looks structurally similar to acetyl CoA. (C) The inhibitor binds to an allosteric region on ATP-citrate lyase and prevents catalysis. (D) You can distinguish between an uninhibited and inhibited enzyme by comparing the x-intercepts on a Lineweaver-Burk plot. (E) This inhibitor lowers the KM of ATP-citrate lyase

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter27: Metabolic Integration And Organ Specialization

Section: Chapter Questions

Problem 20P: Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy...

See similar textbooks

Related questions

Q: In the complete b-oxidation of stearic acid (18:0) How many moles of acetyl CoA are produced? How…

A: Fatty acid oxidation is often referred to as beta-oxidation because, at the beta-carbon position…

Q: . Consider the oxidation of the fatty acid with the common name arachidic acid. a. Draw the…

A: Arachidic acid is a 20 carbon saturated fatty acid. Beta oxidation is oxidation of fatty acid at…

Q: what is the key enzyme of the pentose phosphate pathway also serving as the dirst enzyme of the…

A: The initial metabolite of the pentose phosphate pathway is glucose-6-phosphate, 2 NADP+, and H2O.…

Q: What structurtal property of mono- and polyunsaturated fatty acids prevents oxidation of these…

A: Fatty acids are important lipid molecules. They are characterized by the presence of a carboxylic…

Q: Account for the number of ATPs produced when other monosaccharides like galactose, fructose, and…

A: In most organism hexoses other than glucose can undergo glycolysis after conversion to a…

Q: The following reaction is a vitamin B12-dependent reaction that proceeds via a mechanism analogous…

A: Methylmalonyl-CoA mutase is encoded by gene MUT, it is a vitamin B12 dependent enzyme that catalyzes…

Q: Regarding phosphofructokinase, which of the following statements is true

A: Phosphofructokinases are kinase group of enzyme that phosphorylates fructose-6 phosphate by…

Q: Many NADH-linked dehydrogenases have similar active sites. Which part of glyceraldehyde-3-phosphate…

A: Glyceraldehyde 3-phosphate dehydrogenases (GAPDHs) are glycolytic enzymes, cytoplasmic in nature. It…

Q: Which of the following biochemical conversions can be carried out by the least number of proteins?…

A: Cellular respiration is a metabolic process that take place in the cells of organisms, which…

Q: Certain microorganisms with a modified citric acid cycle decarboxylate α-ketoglutarate to produce…

A: The citric acid cycle also known as TCA cycle. It is a series of chemical reactions used by all…

Q: If the w-carbon atom of a fatty acyl CoA molecule with a C18 chain is labeled with C14, how many…

A: Fatty acids consist of an carboxylic acid group at one end of the molecule, thus making it an acid.…

Q: Step 2 of B-oxidation involves a reaction with chemistry characterized as a dehydration. B-Oxidation…

A: Introduction: The oxidation of fatty acid on the β-carbon atom is beta-oxidation. It occurs in the…

Q: For each of the central pathway intermediates given below, calculate how many ATP equivalents can be…

A: All of the given molecules will be converted to carbon dioxide only in the presence of oxygen. Given…

Q: Consider a preparation that contains all the enzymes and cofactors necessary for fatty acid…

A: Questions 1 - This process takes place in the cytoplasm of the cell. Most of the acetyl-CoA which is…

Q: Propose a mechanism for the conversion of glucose 6-phosphate into fructose 6- phosphate by…

A: Glycolysis is the oxidation process of glucose to yield high-energy compounds such as ATP . The…

Q: Consider the dextran sucrase reaction. Why do you suppose there is not an ATP requirement to…

A: Dextran sucrase is an enzyme that catalyses the chemical reaction.

Q: For beta-oxidation of an 18:1 oleate mono-unsaturated fatty acid: - How many Acetyl CoA produced? -…

A: Fatty acids are molecules with hydrocarbon chain and carboxylic acid group. In the absence of…

Q: One of the regulators of the TCA cycle is succinyl CoA. Discuss the rationale for this molecule to…

A: The TCA cycle is a vital biochemical process in the glucose catabolism pathway. The TCA cycle is…

Q: In a biochemical pathway where A--1--B--2--C--3--D. If D is a competitive inhibitor of enzyme 1,…

A: Answer: Introduction: Competitive inhibition is break down of a chemical pathway due to one chemical…

Q: Pyruvate can be processed under anaerobic conditions to ethanol (in yeast) or to lactate (in…

A: Introduction: Pyruvate is the product of glycolysis which is an important junction point in…

Q: Anabolic reactions like fatty acid synthesis are inherently endergonic and require exergonic driving…

A: The synthesis of fatty acids mainly palmitic acid from acetyl CoA involves seven enzyme reaction,…

Q: Mechanistically, how does NADPH facilitate the removal of an oxygen atom from intermediates in fatty…

A: Fatty acid synthesis is the production of fatty acids from acetyl-CoA and NADPH through the action…

Q: Although animals cannot synthesize glucose from acetyl-CoA, if a rat is fed 14C-labeled acetate,…

A: Muscles are defined as the soft tissue that is responsible for bringing movement in different body…

Q: Linking a molecule of acetate to CoA is an example of what type of common biochemical reaction? ATP…

A: Acetyl CoA participates in the reactions of carbohydrates, lipids, and proteins. The main function…

Q: What is the total energetics involved in the complete beta-oxidation of one mole of 19C fatty acid…

A: ATP (adenosine triphosphate) is the main energy currency of a living cell. The cell produces the ATP…

Q: If the carbonyl carbon of acetyl-CoA were marked with 14C, where would that carbon be located within…

A: Metabolism includes biosynthesis/ reduction (an anabolic process) and oxidation (catabolic…

Q: Starting with acetyl-S-enzyme-1 and malonyl-CoA, how many molecules of acetyl-CoA synthesize an…

A: * Acetyl CoA is a molecule that involves in many biochemical reactions like protein and…

Q: succinyl CoA?

A: The tricarboxylic acid cycle (TCA) cycle is a series of eight catalyzed reactions as well as eight…

Q: Consider the following mechanism: What kind of reaction is occurring during this step of glycolysis?…

A: "Since you have asked multiple subparts questions, we will solve the first five question for you. If…

Q: The conversion of glucose-6-phosphate to fructose-6-phosphate in the glycolytic pathway (Choose all…

A: Glycolytic pathway is the metabolic pathway which involves a sequence of enzymatic reactions that…

Q: What is the quaternary structure of glycogen phosphorylase? How would you characterize the…

A: The allosteric effect is generally the binding of a ligand to one part on a protein molecule and…

Q: Glyphosate, which inhibits the EPSP synthase reaction of the shikimic acid pathway, is a phosphonic…

A: The biochemical reaction to combine PEP and 3-phosphoshikimate (S3P) to give phosphate and…

Q: Human beings contain two different carbamoyl phosphate synthetase enzymes. One uses glutamine as a…

A: The ATP-dependent synthesis of carbamoyl phosphate from glutamine or ammonia is catalyzed by…

Q: In the hexokinase reaction (in glycolysis) Keq caclualtion, what is the OBVIOUS problem with getting…

A: Cellular respiration is a catabolic pathway of the process of metabolism, where a series of chemical…

Q: Which of the following statements is TRUE about cholesterol biosynthesis? Group of answer choices…

A: Cholesterol biosynthesis pathway involves the conversion of the Acetyl CoA to the Acetoacetyl CoA…

Q: Which one of the following statements does NOT correctly describe the current state of the…

A: Chymotrypsin is synthesized in the pancreas. Chymotrypsin is the serine protease. In the active site…

Q: Discuss how hypoglycin (an unusual amino acid found in the unripened fruit of the akee tree) acts as…

A: Hypoglycin is responsible for the stimulation of inhibitors of fatty acids oxidation. The ingestion…

Q: If oxidation of acetyl-CoA yields 10 ATPs per mole through the citric acidcycle, how many ATPs will…

A: The citric acid cycle is the oxidation of acetyl-CoA to produce ATP. It also provides an entry point…

Q: Consider docosanoic acid C12H43CO2H a. Label the alpha and beta Carbons. Show the beta-oxidation in…

A: Beta oxidation is a process where the fatty acids are metabolized to make energy. It is a catabolic…

Q: What is the likely substrate of α ketoglutatrate dehydrogenase? A) water B) alpha ketoglutatrate C)…

A: Tri carboxylic acid cycle is a amphibolic pathway that means it involves both catabolic and anabolic…

Q: When regulating phosphofructokinase, why is there such extensive regulation of this reaction and…

A: Glycolysis is a metabolic pathway that involves breaking down glucose to produce pyruvate and…

Q: We have encountered reactions similar to the oxidation, hydration, and oxidation reactions of fatty…

A: Beta oxidation is a metabolic process in which fatty acid molecules are broken down to create energy…

Q: Amount (in μmol) 120 100 80 60 40 20 0 0 50 100 150 200 Time (s) 250 300 350

A: Salivary amylase is the Enzyme that acts on starch to break them into glucose units. Starch---->…

Q: Which of the following biochemical conversions can be carried out by the least number of proteins?…

A: Fatty acid synthesis is a major anabolic pathway in organisms. They are important component of…

Q: Where in the biochemical pathway of cellular respiration (glycolysis, intermediate stage, or citric…

A: Cellular respiration is the metabolic process where biomolecules such as carbohydrates, lipids, and…

Q: Consider the complete oxidation of one mole of arachidic acid (20:0). ___________ a. How many…

A: Beta-oxidation is the process that is responsible for the breakdown of fatty acids. Beta-oxidation…

Q: Most individuals with genetic defects in oxidative phosphorylation are found to have relatively high…

A: Genetic defects in the oxidative phosphorylation are known to reduce the synthesis of ATP, which…

Question

You are studying the kinetics of a novel competitive inhibitor of ATP-citrate lyase that can be used to prevent obesity. What is likely true about this inhibitor? (A) This inhibitor lowers the Vmax of ATP-citrate lyase. (B) The inhibitor likely looks structurally similar to acetyl CoA. (C) The inhibitor binds to an allosteric region on ATP-citrate lyase and prevents catalysis. (D) You can distinguish between an uninhibited and inhibited enzyme by comparing the x-intercepts on a Lineweaver-Burk plot. (E) This inhibitor lowers the KM of ATP-citrate lyase. Related to this question, you should also be able to: • explain the function of ATP-citrate lyase and why inhibition would prevent obesity • interpret Michaelis-Menten & Lineweaver-Burk plots related to this question • explain the differences between a competitive and non-competitive inhibitor

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step 5

Step 6

Step 7

Trending now

This is a popular solution!

Step by step

Solved in 7 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Similar questions

Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy charge. a. Would hexokinase be an R enzyme or a U enzyme? Would glutamine: PRPP amidotransferase, the second enzyme in purine biosynthesis, be an R enzyme or a U enzyme? b. If energy charge = 0.5: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low? c. If energy charge = 0.95: Is the activity of hexokinase high or low? Is ribose-5-P pyrophosphokinase activity high or low?
Using the ActiveModel for aldose reductase, describe the structure of the TIM barrel motif and the structure and location of the active site.
Draw a schematic illustration of the hydrolysis of N-acetylphenylalaninamide catalyzed byalpha-chymotrypsin in which you indicate the important catalytic residues in the active site and how thesubstrate undergoes transformation to products through two different tetrahedral intermediates.
Looking at the structure of alpha linolenic acid, how many rounds of beta oxidation will occur to fully oxidize the fatty acid into acetyl CoA molecules?
Which of the following biochemical conversions can be carried out by the least number of proteins? Assume proper metabolic conditions, abundance of cofactors, necessary accessory molecules, etc. 1 propionyl-CoA → 1 succinyl-CoA 1 palmitate (16:0) → 8 acetyl-CoA 1 acetyl-CoA + 7 malonyl-CoA → 1 palmitate (16:0) 1 palmitate (16:0) → 1 acetyl-CoA + 1 myristic acid (14:0) 1 acetyl-CoA → 1 succinate Please answer very soon will give rating surely
One of the regulators of the TCA cycle is succinyl CoA. Discuss the rationale for this molecule to be used to regulate the TCA cycle [include chemical structures and chemical equations where appropriate]. What is an allosteric inhibitor? How does it operate? For what TCA enzymes does succinyl CoA act as an inhibitor? What is the metabolic role of succinyl CoA? So then why is this molecule a reasonable choice as an inhibitor of the TCA?
Why does it make metabolic sense for UTP to inhibit carbamoyl phosphate synthetase II, whereas ATP activates the enzyme?
Mechanistically, how does NADPH facilitate the removal of an oxygen atom from intermediates in fatty acid synthesis?
UDP-glucuronosyltransferase enzymes bind the organic compound UDP-glucuronic acid (UDP-GA) in order to catalyse the transfer of a glucuronic acid group from UDP-GA to a drug molecule, releasing UDP from the active site as a product. UDP is then regenerated by the activity of another enzyme. What terms could be used to describe UDP-GA?
Consider the following mechanism: What kind of reaction is occurring during this step of glycolysis? What metabolite is in Box 1? What metabolite is in Box 2? What metabolite is in Box 3? What enzyme catalyzes this reaction? Circle and label one place on the mechanism where covalent catalysis is occurring. Suggest an amino acid that could fill the role of residue “A” in the mechanism above and draw its structure at pH 7.4. Suggest an amino acid that could fill the role of residue “B” in the mechanism above and draw its structure at pH 7.4. Under standard conditions, this reaction is unfavorable (DG¢° = 23.8 kJ/mol). What conditions in the cell allow for the actual free energy change to be lower (DG » 0), making the reaction readily reversible? Explain your answer. *please help and explain as well as you can*
Using the principles described in the text regarding pyridoxal phosphate mechanisms, propose a mechanism for the reaction catalyzed by serine hydroxymethyltransferase.
Write a balanced equation for each of the following reactions or reaction sequences. (a) The reaction catalyzed by PFK-2 (b) The conversion of 2 moles of oxaloacetate to glucose (c) The conversion of glucose to UDP-Glc (d) The conversion of 2 moles of glycerol toglucose (e) The conversion of 2 moles of malate to glucose-6-phosphate