The techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to: Staining the unknown for initial characterization by microscopy. Using a dichotomous key strategy to systematically rule out other organisms. Testing the organism for key biochemical traits.

The techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to: Staining the unknown for initial characterization by microscopy. Using a dichotomous key strategy to systematically rule out other organisms. Testing the organism for key biochemical traits.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 9RQ

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You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques that can be used to identify unknown bacteria (you can use any reliable online resources). Note: These must not be based on biochemical tests (enzyme use, acid production etc) or morphological characteristics (size, shape, structures).
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Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).
Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. DO NOT simply copy the dichotomous key from you lab manual or from other sources (e.g. Bergey’s manual). The organisms are not the same as the ones used in this exercise. Also, because of genetic variability between organisms, the results may not be same as in our lab book or in other sources. Use the tables in your lab notes to create your key. The key must include a source for each test. You can use footnotes in your key or just include the information right next to test name. Since the tests…
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