You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
Chapter14: Formula Method
Section: Chapter Questions
Problem 33SST
Related questions
Question
100%
You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
Transcribed Image Text:You assay 0.5 ml of 1/2000 diluted egg
Absorbance @ 595 nm
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0
2
4
Bradford calibration curve
6
8
10
12
14
µg protein in assay (Bovine Serum Albumin)
Bradford calibration curve
16
18
20
SUBMIT
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
Recommended textbooks for you