How to calculate the Km based on this data?
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How to calculate the Km based on this data?
| Vo (umol/min) |
Substrate added (mmol/L) |
| 217 | 0.8 |
| 325 | 2 |
| 433 | 4 |
| 488 | 6 |
| 650 | 1000 |
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- What percentage of max is obtained when the substrate is present at 80% of the Km? Use two digits in your answer.Determine the fraction of Vmax that would be obtained when the substrate concentration, [S], is 0.5Km. Vmax fraction: .769Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?
- Complete the table by calculating the theoretical yield of SLT-88 and the percent yield of SLT-88. Round your amounts to the nearest milligram and your percentages to the nearest whole percent.Use Beer Lamberts law to calculate the concentration of oxidised DCPIP A= 600 L = 1cm K = 11800 M^-1fill in the volumes required to make the various dilutions of Coomassie Blue and their respective concentrations. show sample calculations.
- What is the purpose of the respirometer with just rocks (the thermobar)? What conditions would cause movement of the water bubble in the pipette of this respirometer during the experiment?Using the data in this table, what is the AG° (in KJ/mol) for the reduction of FAD by water?3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
- Derive a general expression for the minimum filter area requirement per a sterility assurance limit (SAL) in terms of(a) concentration of microorganisms in the feed; (b) volume per unit parenteral dose; (c) sterility assurance limit; and (d) filter capacity.Compute for the CFU/ml of drinking water. Is this Passed or Failed based on the Philippine National Standards for Drinking Water for Heterotrophic Plate Count (HPC)?You are given as following : 20 µl pure LDH on ice, 2.0ml of 6mM NAD+, 2.0ml of 150mM lactate, and 0.14M CAPS buffer. LDH reaction cocktail has final concentration of 1mM NAD+ and 25mM lactate in 0.14M CAPS buffer. LDH activityis measured by mixing 10 µl of LDH sample and 990 µl LDH reaction cocktail before getting ∆A340/min reading on spectrometer. (a). Describe in detail how you would prepare for your LDH reaction cocktail including how to make dilutions.