You conduct an experiment to study the expression of the S protein through the tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment you inject in the leaves a messenger RNA that codes for the synthesis of a single protein made of two parts that are attached to each other: the functional S protein and a red fluorescent protein tag (RFP). You perform two cross sections of the same root, one section at the start of the experiment (time = Oh; corresponding to the time of injection) and one section in the afternoon (time = 8h). Through fluorescent microscopy you observe a change in the coloration inside the cells of the root's central tissues (as indicated by the arrows): from no coloration (time = Oh) to red (time = 8h). • The cells of the central tissues in the roots do not have nuclei or ribosomes. How can you explain this change of coloration? Please provide a cellular feature that can lead to this. • What is one advantage of using an RFP-tag in an mRNA? mRNA S-gene RFP-tag 3
You conduct an experiment to study the expression of the S protein through the tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment you inject in the leaves a messenger RNA that codes for the synthesis of a single protein made of two parts that are attached to each other: the functional S protein and a red fluorescent protein tag (RFP). You perform two cross sections of the same root, one section at the start of the experiment (time = Oh; corresponding to the time of injection) and one section in the afternoon (time = 8h). Through fluorescent microscopy you observe a change in the coloration inside the cells of the root's central tissues (as indicated by the arrows): from no coloration (time = Oh) to red (time = 8h). • The cells of the central tissues in the roots do not have nuclei or ribosomes. How can you explain this change of coloration? Please provide a cellular feature that can lead to this. • What is one advantage of using an RFP-tag in an mRNA? mRNA S-gene RFP-tag 3
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Transcribed Image Text:You conduct an experiment to study the expression of the S protein through the
tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment
you inject in the leaves a messenger RNA that codes for the synthesis of a single
protein made of two parts that are attached to each other: the functional S protein
and a red fluorescent protein tag (RFP). You perform two cross sections of the same
root, one section at the start of the experiment (time = Oh; corresponding to the time
of injection) and one section in the afternoon (time = 8h). Through fluorescent
microscopy you observe a change in the coloration inside the cells of the root's
central tissues (as indicated by the arrows): from no coloration (time = Oh) to red
(time = 8h).
• The cells of the central tissues in the roots do not have nuclei or ribosomes.
How can you explain this change of coloration? Please provide a cellular
feature that can lead to this.
• What is one advantage of using an RFP-tag in an mRNA?
mRNA
S-gene
RFP-tag 3
Time 8h
Time = Oh
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