You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold.
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- How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL?How would you prepare a solution with a 1:500 dilution of your antibody in a 4mL volume of blocking buffer?Suppose you were instructed to make two antiserum solutions: The first is identical to what you used in the lab exercise. The other is a 10:6 dilution of the antiserum. After incubation with the antigen, the full-strength antiserum produces a precipitous ring, but the diluted antiserum does not. Explain these results. Is this due to poor specificity or sensitivity of the test?
- The first table is known, and the second is unknown. we are trying to determine by ELISA if unknown samples (1 & 2) contain antibodies for chicken gamma globulin antigen, which sample contain the antigen? •Create Fig 1 -Draw a standard curve using the semilog graph paper. Remember, your X-axis is your absorbance at 655nm (linear scale), and your Y-axis is your concentration in ng/ml (log scale) •Show the concentrations of unknowns (how you find them by drawing vertical lines) in Fig 1. Please label the negative and positive control and the unkown 1 and 2.How would you determine the optimal antigen: antibody ratio by means ofthe ring test?Mangaba, the laboratory technician, is interested in determining narcotics in saliva at this concentrations > 10 mg/L (μg/mL). One approach being considered is to use an immunochemical assay. Which immunoassay would be most suitable for the desired assay? Explain. Describe the immunoassays that are sensitive enough to measure as little as 10 mg/L of antigen? Which of these assays can quantitate urine albumin? Which of these assays are technically complex or require dedicated, expensive instrumentation?
- You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody reactivity is enhanced by acidification?The order is for Streptomycin 1gm IM. You have a 5gm vial of Streptomycin . The label states to add 9ml of sterile water to yield 400mq / m * l How many ml will you give?Why do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?
- True or false 1. Heterogenous antibodies used for structural studies can be obtained by monoclonal hybridoma technique. 2. PrP(Sc) is largely alpha-helical structure.Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three secondary antibodies. Why? I understand that secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody which directly binds to the target antigen. However, I don't undrstand why there are only two or three secondary antibodies available in the laboratory. Glad if the expert would advise.You just received the properly labeled blood bank specimen on patient Aran Stark. You decide to collect some background information about her known historical antibodies before beginning the work-up knowing that she has a history of anti-E, anti-K, anti-Jk^a, anti-Fy^a, anti-M and anti-Le^a. Which antibody can be neutralized? Which antibody is destroyed with 0.2M DTT treatment? Which antibody reactivity is enhanced by acidification? Which of the antibodies that are typically IgG in nature are destroyed by enzymes? Which are enhanced by enzymes? Which of these antibodies have been known to cause hemolytic transfusion reaction? Which of these antibodies are known to react at room temperature? Which of these antibodies react best at 37C? * When you complete the work-up, you note that the anti-Jk^a antibody is no longer detectable. Can the patient receive red blood cells that contain the Jk^a antigen? Why or why not?