You have carried out an experiment using the spectrophotometry concept. A solution containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a blue colour whose absorbance (A) could be read at 550 nm. The results are shown below If the standard solution (compound X) used have a concentration of 1 mM:  3. If the absorbance was read using 1 cm diameter cuvettes, calculate the molar extinction coefficient for compound X in the solution. State the unit for the coefficient. Assume each molecule of X produces one molecule of coloured compound

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
Publisher:Crouch
Chapter14: Chromatography
Section: Chapter Questions
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You have carried out an experiment using the spectrophotometry concept. A solution
containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a
blue colour whose absorbance (A) could be read at 550 nm. The results are shown below

If the standard solution (compound X) used have a concentration of 1 mM: 

3. If the absorbance was read using 1 cm diameter cuvettes, calculate the molar extinction
coefficient for compound X in the solution. State the unit for the coefficient. Assume
each molecule of X produces one molecule of coloured compound

Test
tube no.
2
3
4
5
6
7
Sample
Y
Standard
solution X
(ml)
0
0.1
0.2
0.4
0.6
0.8
1.0
1.0
Reagent 1 Reagent 2
(ml)
(ml)
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
Water (ml)
1.0
0.9
0.8
0.6
0.4
0.2
0.0
0.0
A550 nm
0.0
0.05
0.12
0.24
0.35
0.48
0.59
0.35
Transcribed Image Text:Test tube no. 2 3 4 5 6 7 Sample Y Standard solution X (ml) 0 0.1 0.2 0.4 0.6 0.8 1.0 1.0 Reagent 1 Reagent 2 (ml) (ml) 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 Water (ml) 1.0 0.9 0.8 0.6 0.4 0.2 0.0 0.0 A550 nm 0.0 0.05 0.12 0.24 0.35 0.48 0.59 0.35
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