You need to resuspend lyophilized protein in a solution of 5mM HEPES with 20% glycerol. Describe how you would make 1mL of this solution. HINT: you can make a greater volume of 5mM HEPES.
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You need to resuspend lyophilized protein in a solution of 5mM HEPES with 20% glycerol. Describe how you would make 1mL of this solution.
HINT: you can make a greater volume of 5mM HEPES.
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- In order to metabolize lactose, most infants express the enzyme lactase in their intestines. What kind of glycosidic linkage does lactose have? Please draw the individual monosaccharides to explain your answer. Please explain how the monosaccharides in lactose will be used in glycolysis. Be specific.Penicillin reacts with the cysteine residue of penicillin binding protein. Show the reaction here.What type of protein is included in milk? When milk sourness occurs, why does this protein precipitate?Discuss the overall process or mechanisms that cause proteins to precipitate.What is the definition of amphoterism? Show the amphoteric property of protein using a chemical equation. Strictly no plagiarism. Please cite the references.
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- If an octapeptide IDEWLVAS is digested with chymotrypsin enzyme, which column would you use? Chymotrypsin cleaves on the carboxy side of bulky and aromatic amino acids. A. Sephadex column B. None of the above C. Anion Exchange column D. Cation Exchange column Please explain why.Proteoglycans are often made up of glycosaminoglycans (chondroitin sulfate, keratan sulfate, and dermatan sulfate) that are covalently linked to a core protein. True Falsegive two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.