You perform a thin-layer chromatography test on pigments extracted from a purple flower. There are three spots, a red spot with an Rf of 0.32, a blue/purple spot with an Rf of 0.57, and an orange spot with an Rf of 0.97. Explain the intermolecular forces these pigments are likely to have those placements on the TLC plate.

You perform a thin-layer chromatography test on pigments extracted from a purple flower. There are three spots, a red spot with an Rf of 0.32, a blue/purple spot with an Rf of 0.57, and an orange spot with an Rf of 0.97. Explain the intermolecular forces these pigments are likely to have those placements on the TLC plate.

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter91: Gas Chromatography

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

1. What is paper chromatograpy?2. What are the stationary phase and mobile phase in paper chromatograpy?3. What is the principle underlying the paper chromatograpy?4. What is Rf value of a dye or pigment?5. Would you expect that changing the solvent will change the Rf value of the specific solute? Why?6. Suggest simple reasons why the Ni2+ and Fe3+ ions have different Rf values?7. What forces causes the eluting solution to move the chromatographic support material?
a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?
thin layer chromatography: What would happen to the Rf value of a compound if you increased the polarity of the mobile phase while using a polar stationary phase? please help me this question! Thank you a lot
The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solvent
A student has a green dye mixture on which she performs a TLC experiment with a silica gel plate and acetone as the solvent. She obtains a yellow spot with an Rf of 0.75 and a teal spot with an Rf of 0.45. Explain the significance of her data.
Thin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!
a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used as examples to explain difference between adsorption and partition. For each of these chromatographic techniques, identify: + the stationary and mobile phase + how the mobile phase moves b) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)
a 150 g. dried leaves sample of Ficus religiosa was subjected to ethanol extraction for 48 h, an empty round bottom flask was used in the rotavap weighing 168.77 g. After removing the ethanol solvent, the round bottom flask weighted 172.49g. explain the principle of chromatography using the terms, partition, adsorption, solubility, polar and non-polar, mobile phase and stationary phase.
1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.
We use reverse phase column chromatography with a non-polar C18 stationary phase and a mixture of acetonitrile and methanol as mobile phase in order to separate the dye molecules methyl fuchsin, parafuchsin, basic fuchsin and malachite green. What will be the order of elution of these molecules. Explain. Why won't these compounds separate using normal phase chromatography with silica and acetone?
1- What are the main two types of columns in the HPLC? 2-What are the most disadvantages of mass-spectrmeter as a detector for HPLC? 3-What Are The Commonly Used Carrier Gases In GC Analysis ? 4-Name four of the mechanisms of the separation in chromatography? 5-Which Is The Most Commonly Used Detector In High Performance Liquid Chromatography And Why?