You used a mobile phase of 90% ethyl acetate (CH3CH2COOCH3)/5% acetic acid (CH3CO2H) as the mobile phase to develop the TLC plate that was spotted with analgesic compounds. What would have been the appearance of the developed plate if a mixture of 80% ethyl acetate and 20% acetic acid were used as the mobile phase? O The order of elution of the compounds would be reversed. O All the compounds would not have traveled as far up the plate. O All the compounds would have traveled farther up the plate. O Some of the compouunds would have migrated farther and some would not have migrated as far.

You used a mobile phase of 90% ethyl acetate (CH3CH2COOCH3)/5% acetic acid (CH3CO2H) as the mobile phase to develop the TLC plate that was spotted with analgesic compounds. What would have been the appearance of the developed plate if a mixture of 80% ethyl acetate and 20% acetic acid were used as the mobile phase? O The order of elution of the compounds would be reversed. O All the compounds would not have traveled as far up the plate. O All the compounds would have traveled farther up the plate. O Some of the compouunds would have migrated farther and some would not have migrated as far.

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter8: Thin-layer Chromatography: Analyzing Analgesics And Isolating Lycopene From Tomato Paste

Section: Chapter Questions

Problem 10Q: A TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the...

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A TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the developing chamber, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber. What would you expect to see after the second development was complete?
A mixture of biphenyl, benzyl alcohol, and benzoic acid is spotted on a silica gel TLC plate and developed using Ethyl Acetate with 0.5% acetic acid as the solvent. Predict the relative Rf (highest to lowest).
How would you expect the result of TLC plate with the crude extract, phospholipid extract and non- phospholipid extract, olive oil, cholesterol, oleic acid. with the mobile phase of hexane/diethyl ether/acetic acid; 60/40/2; v/v/v. mobile phase 2: iso-propanol / cyclohexane (1:1.25, v/v) phase mobile 3: chloroform/methanol (2/1, v/v)? please suggest it with reasonings!
The three TLC plates below show the analysis of the same mixture of two compounds. One plate was developed in 100% DCM, one in 90:10 DCM/MeOH, and one in 80:20 DCM/MeOH. Determine which plate was developed in which solvent.
is hexane or 70:30 hexane: ethyl acetate a better eluent based on their TLC plates and rf value in column chromatography separation of ferrocene-acetylferrocene.
Two compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answer
Extraction of the aqueous salicylic acid solutions with 10% ethyl acetate/hexane (density ~ 0.7 g/ml) will give two layers in the separatory funnel. Will the aqueous layer be the upper layer or the lower layer? If dichloromethane (density ~ 1.3 g/ml) were substituted for the 10% ethyl acetate/hexane solution, which layer would be the aqueous layer?
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You spotted a mixture of three compounds, labeled A-C, on a silica plate. The resulting thin layer chromatography (TLC) plate with a mobile solvent mixture of 95 % hexanes and 5 % ethyl acetate is shown below. Which of the spots traveled the furthest up the TLC plate corresponding to spot 3?
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The label is a reagent to which he had specified on the label only the alcohol name and molecular formula of the same which was C4H10O. At the beginning of the experiment, about 50 mg reagent was diluted in deuterated chloroform with standard internal TMS and then added a specific vial (tube) for analysis of 1H and 13C spectra. When operating the equipment and ending the experiment, the teacher of discipline two being a spectrum of each 1H and one of 13C, and requests that they indicate which structure was responsible for those chemical spectra. Do you know those hired to the students who are here, qualify the structure of the referred responsible for the set of 13C and 1H spectra, as requested by the teacher. Figure 1: 1H spectrum of the unknown Figure 2: 13C spectrum of the unknown reagent