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- A Er doctor orders IV normal saline solution to be infused at a rate of 140mL/hour for a patient admitted with mild dehydration and bronchitis. How many liters of saline will the patient receive during a 12 hour shift?You are given a buffer that is labeled 10x and your instructor asks you to dilute it to 1x in the final tube. If you have a final volume of 100uL in your tube how much of the 10x buffer will you need to add to bring it to 1x? Do you need to add anything else to the tube before using the new solution?