Enzymes are central to every biochemical process. Due to their high specificity they are capable of catalyzing hundreds of reactions that signifies their vast practical importance. This experiment was conducted to investigate the enzymatic activity of the enzyme aldolase by using different urea concentrations. Stronger solutions of denaturant in this case urea were used to increase the polypeptide subunit unfolding and the dissociation of the tetramer. Aldolase is an enzyme that consists of four
Relating Chemical Systems and Equilibrium to Technology, Society, and the Environment An increase in average global temperature/an increase in extreme weather conditions, such as hurricanes and tornadoes Is the carbon cycle involved? Recent scientific evidence indicates that humans have upset the carbon cycle, leaving more carbon dioxide and other greenhouse gases in the atmosphere. Burning fossil fuels such as oil and natural gas, burning biomass such as ethanol and wood, creating cement and releasing
Spectrophotometric Determination of an Equilibrium Constant. Abstract: The report presents determination of equilibrium constant for the formation of a complex ion FeSCN2+. This was accomplished using a colorimeter to measure absorbance of some known concentration solutions in order to generate the calibration curve. The equation of the graph was used to compute the equilibrium concentrations of the reactants and products, needed to calculate the equilibrium constant for the reaction.
Determination of an Equilibrium Constant Abstract: In this experiment, two reactions were run to determine the molar absorptivity and the equilibrium constant of FeSCN2+. The main principles used in this lab are equilibrium, LeChatlier’s Principle, Beer’s Law and Spectrocopy. The first reaction was run to completion using LeChatier’s Principle and the second reaction was run to equilibrium. A spectrophotometer was used to measure absorbances. Using a graph of absorbance versus concentration
determine an equilibrium constant for specific reactions by using Beer's law and Le Châtelier’s principles. Reactions that are at equilibrium are constantly occurring in both directions. In order to test the consistency of the equilibrium constant we will change concentrations of the reactants to see how that affects equilibrium. Introduction Equilibrium can be reached when the forward and reverse reactions are occurring at the same rate. To determine if a reaction is at equilibrium, you can record
DATE PERFORMED: JULY 20, 2007 SPECTROPHOTOMETRIC DETERMINATION OF EQUILIBRIUM CONSTANT FOR A REACTION ABSTRACT UV-VIS spectrophotometry is one of the most widely-used methods for determining and identifying many inorganic species. During this experiment, this spectrophotometry was used to determine the equilibrium constant, Keq, of the Fe3+(aq)+SCN-(aq)↔ FeSCN2+(aq) reaction. By determining the amount of light absorbed, the concentration of the colored FeSCN2+ solution was also quantitatively
Reaction of Iron (III) Nitrate and Potassium Thiocyanate Analysis When the light yellow iron (III) nitrate solution was added to the colorless potassium thiocyanate solution, the color of the final solution was red. To understand why this happens, the individual reactants must be examined. The potassium thiocyanate solution can be broken down as follows: KSCN(aq) ↔ K+(aq) + SCN–(aq). Both the potassium ions and thiocyanate ions are hydrated with water molecules. Similarly, the iron (III) nitrate
Baltazar Chemistry 73 (Laboratory) August 11, 2014 Simultaneous Determination of Several Thermodynamic Quantities: K, ∆G°, ∆H°, and ∆S° Feldan P. Villarta I. INTRODUCTION The solubility product constant, Ksp, is the equilibrium constant for a solid substance dissolving in an aqueous solution. It represents the level at which a solute dissolves in solution. A more a substance dissolves, the higher the Ksp value it has. In this experiment, a system of a sparingly soluble
tend to react to form products until a state is reached where the amounts of reactants and products no longer change. Reactants and products are in chemical equilibrium and will remain until the system is altered in some way. Temperature change and the alteration of the concentration of reactants or products disturbs the equilibrium. The equilibrium constant, Keq, expresses the essential condition on the concentrations of reactants and products for the reaction. The Keq can let you know which way
chemistry, equilibrium occurs when both the reactants and products in an equation are present in concentrations which have no further likelihood to change with time. Generally, this state results when the forward reaction proceeds at the same rate as the reverse reaction. Concentration, pressure and temperature are factors that can affect the equilibrium state of a reaction (Source). In 1803, the concept of chemical equilibrium was developed after Berthollet discovered that some chemical reactions