Protein Data Bank

successfully utilized efficiently for characterization, documentation and identification of crop landraces. This type of characterization involves use of biochemical markers which include principally isozymes (metabolic enzymes) and seed proteins which are non-enzymatic proteins (Anderson and McDonald, 1983). The major advantage of biochemical markers is their presumed selective neutrality. This general neutrality allows breeders to distinguish those similarities that are due to common ancestry (Gepts, 1993)

Tannase’s protein purification The cloning, expression, and purification of tannase enzyme obtained from bacterium L. plantarum were conducted as described (Wu et al., 2013). Tannase’s protein crystallization, heavy-metal derived crystals preparation and diffraction pattern collection By using sitting drop vapor diffusion method, the initial apo form of tannase enzyme was crystallized. Then, the initial tannase crystal formed was purified and enlarged by using micro-seeding method (Wu et al., 2013)

While dysfunction of prion proteins remains the most widely accepted etiology of BSE, the USDA suggests there may be two other possible theories that could better explain the manifestation of BSE: the virino theory and the virus theory. However, both theories pale in comparison to

The cholera toxin (also called choleragen) is a protein that activates adenylate cyclase. Being an enterotoxin, this protein affects the membrane permeability of intestinal wall cells (4). The protein was first purified in 1969 and it is composed by two types of subunits, with one being the binding type of subunit (B) and other being the toxic-active type of subunit (A) (5). The B type has a mass of 64 kDa, which is about 67% of the total protein mass. It is composed by five identical

hormones as described in the manufacturer's instructions, at 37°C in a humidified atmosphere of 5% CO2-95% air. Cells were treated with 15d-PGJ2 or Vehicle (Veh) in basal medium for 24 h, nuclear extracts were prepared and protein concentrations were determined using the BCA Protein Assay kit (Pierce, Rockford, IL). PPARδ Reporter Assay — To determine the activation of PPARδ by 15d-PGJ2 a PPARδ specific luciferase reporter assay was performed using PPARδ Reporter Assay System (IB00121; Indigo Biosciences

Salads are usually the go-to food for dieters and healthy eaters. If you look up salad images in food magazines or on the Instagram, you are bound to found tons of delicious looking recipes that are full of colour and freshness. But the creations can often end up looking soggy and flat when you try them at home. Not to mention how some of the salads we eat as ‘health food’ are better classed as ‘junk’. In this article, we’ll help you learn the secrets to a healthy salad that’s worthy of photographing

PanIB cod/heterozygous cod; (e) 2 max (f) (f) Using the data in this question, predict the effects of global warming on cod fish populations. PanIA PanIA (cod) may spread further north / PanIB PanIB (cod) may move/retreat further north; numbers of PanIA PanIA (cod) may increase / frequency of PanIA allele may increase; PanIB PanIB (cod) may become extinct / frequency of PanIB allele may decrease; 2 max [11] IB BANK BIO VERSION 3 MUHD INAAMULHASAN BIN SATHAR MOHD 2012-2014 11

reasons, the high-level visualization of molecules provides help to cut the work performed in HTS. Together, they offer one of the most effective methods in structure-based drug discovery. On the whole, my aim is to simulate molecules to find hidden protein ligand binding sites for the discovery of new drugs. I

Exploring Protein Structure with the Molecular Visualization FirstGlance in Jmol Introduction: The goal of this experiment was to practice using the FirstGlance in Jmol molecular visualization to examine key structural features of proteins. This work is important because protein structure can be related to function, multiple-sequence alignments and evolutionary preservation, and designing drug. FirstGlance in Jmol makes it fairly easy to perceive structure-function relationships in the protein you chose

for cell cycle and DNA replication genes by target scan and KEGG database. Downregulated miRNAs were overexpressed in OS cell lines by transfecting the synthetic mimics/ inhibitors and checked the expression levels of the target protein at RNA level by qRT PCR and at protein level by Immunoblotting. Further cell