Amplification of 16S Ribosomal DNA Sequences using Polymerase Chain Reaction Edwina Abou Haidar, Houssam Al Koussa, Mary AbedAlAhad. Department of Biology, Lebanese American University, Byblos, Lebanon Abstract The 16s rRNA gene sequencing is a widely common amplicon sequencing method used to identify and compare bacteria in a given sample. This method is well established and allows to study phylogeny and taxonomy of complex microbiomes. In this study, an unknown sample of extracted microbial
The History of Western Music Music has been around since the dawn of time, ever since man first inhabited this planet we have learned to communicate in ways other then conventional speaking. Different Cultures all have there own specific way of communicating through music. Music is basically broken into two specific groups Eastern Music and Western Music. Eastern music is mainly derived from the orient and India. While, Western music first emerged from Europe. Western music has developed
between East and West Berlin has been permanently closed. The next day on August 14th 1961, the Brandenburg gate was closed. From here on, Berlin turned into horrific situation. August 15th 1961, on this date the first East German border guard Conrad Schumann had successfully jumped the barbed wire into West Germany. "MANY PEOPLE were standing around, and that was good, because they distracted my colleagues. I was able to swap my loaded sub-machine- gun for an empty
Cyclopropenyl cation (1), the smallest hydrocarbon molecule is a centro-symmetric molecule with a D_3h symmetry. It’s the simplest aromatic species demonstrating aromatic features linked to the delocalization of the two π electrons. It follows the Hückel’s rule, (4n+2) π electron with n = 0. Aromaticity cannot directly be experimentally measured. However, various methods were performed such as computational studies from the Schleyer group^1, which address the energy, structural changes and magnetic
childhood obesity, which is becoming an epidemic in its own right), more patients are turning to surgery as a solution. In 1992 there were 16,200 bariatric surgical procedures in the United States and by 2008 that number had increased to 220,000 (Schumann, 2010). This paper aims at answering the following question: “In obese adults undergoing bariatric surgery, how does opioid-free anesthesia compare to opioid-based general anesthesia and its effect on post-operative outcomes in terms of post-operative
During the romantic era, there were many great composers that made the Romantic period the most innovative in music history. But Felix Mendelssohn is often viewed as a Classical-Romantic composer, whose style paradoxically incorporated elements of formal balance and graceful control on the one hand, and romantic subjectivity and fantasy on the other. Felix Mendelssohn (Jakob Ludwig Felix Mendelssohn-Bartholdy) was a German composer, pianist, musical conductor, and teacher, and one of the most-celebrated
H The Piano Prepared By: Maria Darbinian Prepared For: Professor Daniel Moser Introduction to Humanities DeVry University Online In the 1700’s the piano was invented by Bartolomeo Cristofori in Florence, Italy first introduced to the world as the "pianoforte" meaning “Soft loud”. “In the last quarter of the 18th century the piano had become the leading instrument of the western art of music that still lives on till today as an exotic instrument played by talented people in the
Compare and Contrast Essay William Faulkner’s “A Rose for Emily” was published in 1931. Emily Grierson, who lives with her father in antebellum Southern aristocracy and her father is very protective toward her. Therefore, she could not immerse with any other people beside her father. When her father passed away, she could not accept it still lives alone in her house and refuses to meet anyone. She teaches china painting for the kids and rejects to pay the tax. This story shows the tradition, loneliness
Brief background Strategic planning is a process used by various organizations in defining their strategy and direction. It is also used for decision making purposes affecting the strategy to be used for allocating resources. In order to accurately determine the direction of a given organization, it is critical to understand the organization's current position as well as the possible avenues via which it can effectively pursue a particular objective (Mckeown,2012). Mckeown further noted that strategic
Introduction The music of the 19th and 20th centuries is classified as late Classical and Romantic periods. During this time frame, composers such as Beethoven, Brahms, Chopin, Debussy, Mendelssohn, and Schumann thrived. Throughout the 1800s, music was extremely diverse in composition and performance, and much of the music centered on people’s religious preferences and nationality. In the 1800s, the Romantic Era helped introduce bold and more dissonant pieces, which were longer and more complex