Sol-gel

When examining the purification data in Table 4, we can analyze the different methods of purification of LDH and determine how effective each methods is in order to ultimately purify LDH. Unfortunately, there are two different definitions of purification: one is have a high end yield of the protein, while the other is having a high end concentration without any contaminating proteins. Furthermore, in order to achieve one type of purification, the other one has to be given up. The very first purification

The cation exchange gel samples are labelled IEX 19-22 as these were the tubes with the highest activity as shown in the above table. The other IEX fractions had significantly lower specific activities so were less likely to contain the protein of interest and more likely to contain unwanted proteins than the higher specific activity fractions. Only the higher specific activity fractions were chosen for this reason. All the fractions that were analysed by gel electrophoresis have the same banding

Gel electrophoresis is a gel technique that separates DNA and proteins based on their mass, by means of an applied electrical field that passes through an agarose or polyacrylamide gel. The concentration of agarose in the gel is commonly 0.8% to 1.0%, since agarose is expensive. The gel is embedded on a buffer, pH of 8.3, which keeps the pH of the solution at equilibrium. Assuming that at typical pH, DNA is negatively charged, denatured protein samples are placed in the wells located on the negative

through the polyacrylamide gel. The next step is to put the gel into the electrophoresis module and to run it. It is run until the proteins have almost reached the bottom of the gel. A blue tracking dye is added to the Laemmli sample buffer in order to track the distance in which the proteins travel through the gel. If it is run for too long, the proteins will run off the bottom of the gel and it will mess up your results. Once the protein reach the bottom of the gel, the gel is stained in order to

Blood Exo DNA ProTeck™ Vacutainer blood collection tube for stabilizing extracellular vesicle DNA in a whole blood sample Research Use Only. Store at room temperature (18 to 25⁰C) Catalog # 0019273 9 × 10 mL tubes 0019273 100 × 10 mL tubes Intended Use Blood Exo DNA ProTeck™ is a 10 mL vacutainer blood collection tube for stabilization of extracellular vesicle DNA in a whole blood sample at room temperature for at least 30

Purpose: Gel Electrophoresis is used to separate macromolecules like DNA and RNA by their size, or proteins by their charge and size. In this experiment, I used the Gel electrophoresis to determine the presence of all dyes in a specific dyes mixture. Hypothesis: If all dyes are present in this mixture, then the dyes in the mixture should travel the same direction as the dyes in it towards the positive electrode because the negatively charged dyes travel towards the positive electrode while the positively

A DNA marker (size standard or a DNA ladder) is loaded into the first well of the gel. The fragments in the marker are of a known length so it can be used to help approximate the size of the fragments in the samples. The prepared DNA samples are then pipetted into the remaining wells of the gel. When this is done the lid is placed on the electrophoresis tank making sure that the orientation of the gel and positive and negative electrodes is correct. To separate the fragments, the electrical current

Biology 3601 Biochemistry Gel Filtration Laboratory Pre-Lab Assignment Instructions This Pre-Lab assignment accompanies the laboratory exercise on gel filtration chromatography. The Pre-Lab includes 10 questions. You must complete each question in your own words and show all work as necessary. The answers to each question must be typed, however, you may submit scanned copies of handwritten calculations. This Pre-Lab assignment is worth 40% of the total Lab Submission

band size of the DNA bands influences the distance that which it travels along the gel. The less base pairs within each band, the further it travels on the gel. This is confirmed in table 1 which was created by analyzing Lane 1, the molecular weight markers on agarose gel #2. The distance migrated was compared to the size of each band. Table 1 confirms this assertion as the band with 1517 bp only traveled 25 mm on the gel where as the band

Introduction Gel electrophoresis involves separation and analysis of macromolecules like deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and various proteins. In electrophoresis an electric current is passed through a solution or gel from one electrode to the other. Ions, molecules, and molecular fragments in the solution or gel are drawn to one of the electrodes according to their charge. When charged molecules are placed in a gel, the speed they travel toward the electrode is influenced by