Solubility

What is the purpose of Step 8 in the Procedure? The purpose of Step 8 is to make sure we get the exact mass of the solution. There could be trapped molecules of water in the solution. Heating the solution would evaporate the water molecules. Given the following situations, predict whether the calculated mole ratio of H2O to MgSO4 to will increase, decrease, or remain unchanged. Explain each of your predictions. Some water is present in the crucible when the magnesium sulfate hydrate is added

Experiment 5: Liquid CO2 Extraction of D-Limonene from Orange Rind Lela Gregory Partnered with Madison Pretto TA: Sylvia Singh Introduction Liquid carbon dioxide extraction, also called CO2 liquid extraction for short, is a method of separation and extraction frequently used in the laboratory setting. The processes uses phase changes that occur due to pressure and temperature changes to its advantage to allow for the extraction to occur1. The phase change allows for components of

This experiments was performed in three parts. In Part 1, the organic solution was separated from the aqueous solution. First approximately 0.300 grams of 1,4-dimethoxybenzene, p-tert-butylbenzoic acid and p-tert-butylphenol was weighed and and placed in a 100-mL beaker with 25 mL of Et2O. Then, the solution was quantitatively transferred a 125 mL separatory funnel and added 10 mL of 0.5 M NaHCO3. The solution was shaken and vent until there was no CO2 being formed. The aqueous layers was drained

In experiment 2, we used liquid-liquid extraction to separate a mixture using two immiscible solvents. We made use of extraction, back extraction, neutralization, and melting point technique to figure out the identity of the acid, base and neutral from the combined unknown mixture. There were a couple things that we had to pay close attention to. For example, identifying the organic and aqueous layer as liquid-liquid extraction is usually used to separate organic product from the mixing with the

Lab Report: Glutathione S-transferase Purification Glutathione-S-Transferase Purification: Two micro centrifuge tubes containing the mixture of 100uL Glutathione Sepharose 4B (collected from Prof Miguel) and 500uL of 1X PBS Buffer (made by diluting 1mL of the 10X PBS buffer with 9mL of distilled water) was centrifuged for 1 minute. Removing its supernatant, it was centrifuged again after adding 500uL of 1X PBS Buffer. To this, 300uL of “no IPTG” and 300uL of “+IPTG” Lysate samples (collected from

1. Items needed for this experiment are: 6 petri dishes that have been prepared with nutrient agar and made into plates, then labeled 10-1 through 10-6, 6 test tubes labeled 10-1 through 10-6 then place in test tube rack, sterilize graduated pipet with alcohol, be sure to remove all of the alcohol from pipet, distilled water, 1 disposable cups, 1 yeast packet, permanent marker, paper towels, bleach, safety gloves, goggles, face mask and apron. 2. Disinfect the work area with bleach and paper towels

1) C) a 5-C sugar and phosphate groups Because nucleic acids are polymers of individual nucleotide monomers. Each nucleotide has three parts which are 5 carbon sugar, a phosphate group and a nitrogenous base. 2) A) Glycerol + 3 fatty acids Because 3) B) has changed parts of its structure Because “a steroid hormone that stimulates development of male secondary sexual characteristics, produced mainly in the testes, but also in the ovaries and adrenal cortex.” 4) D) Amino Acids Because in a mammals

The recrystallized cinnamic acid was obtained from the previous lab. It was a white and flaky powder and had a very slight sweet/spicy smell. When weighed, the recrystallized cinnamic acid was only .340 g. The starting amount was .500 g, so the percent recovery was 68.0%. ((.34g/.50g) x 100%)). The low percent recovery may have been due to the addition of too much solvent(book). Last lab, 90.0 mL of solvent was used to dissolve the cinnamic acid, which was the maximum amount directed by the instructor

Discussion 2 A.1 The experimental determined freezing point of the cyclohexane was 6.5 degree Celsius. By averaging the freezing points of trial 1 and 2, which are 6.4 and 6.5 degree Celsius respectively, the freezing point is calculated. A.2. On adding solute, the freezing point of cyclohexane is lowered. A.3. The weighed initial masses for dish #1 and #2 are 0.248 g and 0.102 g respectively. In order to calculate the molar mass for dish 1 and 2, the following calculations

Our task is to find out the number of pre-1982 pennies and post-1982 pennies inside a sealed vial. Firstly, we need to gather all materials that we need: Digital Scale, empty vial, and a sealed vial with pennies inside. If we’re supposed to estimate the number of old pennies and the number of new pennies, we have to measure what both of them weigh. We also needed to measure the weight of the vial itself, that is why we needed an empty vial. After we did the measurements, we found that an old penny