Armadillidium Vulgares Lab Report

There are many differents ways to identify a bacterial unknown and many different situations where identification would be beneficial. One way to identify bacterial unknowns is to perform biochemical tests. In this experiment multiple biochemical tests were done, by performing these tests on the bacterial unknown received the two different bacteria were then identified. The citrate test is done to test the ability of organisms to use citrate as a carbon source. This test uses Simmons citrate agar, the agar contains sodium citrate as the only carbon source and has bromothymol blue as the pH indicator. The organisms that use citrate as a carbon source use the enzyme to transport the citrate into the cell. The cells converts ammonium dihydrogen

After the experiment, I thought about and considered that if the rate of the reaction in which the alka seltzer tablet dissolves is related to the surface area, so by increasing the surface are it will increase the rate of reaction to when the tablet dissolves in the water. By timing the tablet has it dissolved with the water, the water began to fizz up. My hypothesis was proven correct how I said that the surface are of a half crushed tablet will dissolve faster, I had hypothesized right. It had been that the half crushed tablet had dissolved much faster then the other areas. By increasing the surface area the reaction rate of the tablet will dissolve much faster.

Three paper towels A, B, and C where tested on absorbency. This experiment was tested twice. Paper towel A was 26.5 by 28 centimeters. Paper towel B was 26.5 by 28 centimeters. Paper towel C was 15 by 28 centimeters. Scissors were obtained and each paper towel was cut to 13.5 by 15 centimeters. The weight of the three paper towels at this size was 0.9 grams dry for paper towel A, paper towel B weighed 0.8 grams dry, and paper towel C weighed 1.2 grams. Three beakers weighing 12.2 grams each were obtained. Each beaker was filled with 100ml of water with the outcome of them weighing 128.1 grams each. The weight of each paper towel dry was deducted from the weight of each paper towel. Paper towel A was left to soak for seven seconds.

After precisely conducting the experiment and tabulating the results, data for Paraquat toxicity upon P. vulgaris plants can be interpreted over several different parameters. The parameters by which Paraquat toxicity was examined within this experiment involve visual observations, x-ray diffraction, chlorophyll concentrations, protein concentrations, and lastly malondialdehyde (MDA) concentrations on a per mg of protein basis. As stated before Paraquat is very widely used herbicide known to produce superoxide anions leading to chloroplast membrane damage and ultimately a variety of adverse effects upon the host organism, in this case P. vulgaris (Chia et al., 1982).

Acidity increases by ten times for one pH level increase. Soil pH is a major constraint on plant growth. Since plants are the base of the ecosystem, pillbugs may be affected by the pH change as well. Soil acidity can be assisted by addition of certain other plants, like AM fungi, or the addition of basic materials (Kawahara, et. al., 2016). We decided to investigate which pH level pillbugs naturally preferred. We created three different choice chambers that had pH levels of 5, 7, and 9. This experiment will test if pH has an effect on pillbug choice of habitat.We predicted that there will be more pillbugs in the choice chamber with a pH of 7 because a pH level of 5.5 to 7 is considered to be the normal, natural environment of pillbugs. Usually, the pH that pillbugs are found in are 5.5 to 7 (Kawahara, et. al., 2016). In each habitat, the number of pillbugs will be counted every minute for 15 minutes. If a pillbug is in between chambers during the counting, they may be counted for the chamber to which they are

Referring to the experiment`s hypotheses that the A. franciscana prefers light, temperatures between 20-24 ̊ C, and a basic (pH 8) environment; the results regarding the first treatment, light, were initially vague. According to the experiment results, the A. franciscana did not show a clear preference towards light or dark because both sections contained high concentrations of them; the A. franciscana also strayed from the uncovered section. Several factors may shed light on the results such as the A. franciscanas physical appearance; they possess three light-sensitive eyes that can adjust to both low and high light intensities (Fox, 2001). This means that although they may prefer light they can survive in darker habitats as well; relating back to the experiment the A. franciscana may have been content with wherever they were, resulting in limited movement.

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

Purpose: The purpose of this lab is to observe the behavior of the Armadillium vulgare (roly- poly

In this lab, the organism that we have been working with is the bacterium, Serratia marcescens. S. marcescens is a member of the Enterobacteriaceae family, and tends to grow in damp environments. S. marcescens is an ideal bacterium to work with in the lab because it reproduces quicker than other bacterium. This bacterium produces a special pigment called prodigiosin, which is red in color. The prodigiosin pigment is intensified when S. marcescens is grown at higher densities. During our experiment, temperature, pH, salinity concentration and oxygen requirements were tested on S. marcescens to measure their optimal growth and prodigiosin production.

To perform this test, a tube of broth rich with glucose is acquired. In this tube is phenol red, a pH indicator. Initially, the tube appeared pink in color, indicating a normal pH level. Next, a sample of unknown #44 is introduced into this medium using the aseptic technique, and this is allowed to sit for several days. If the organism is able to ferment glucose, the pH in the medium would decrease and cause the phenol red to exhibit a yellow color. In addition to the straw color, gas can also be produced and trapped inside the Durham tube placed in the medium. This production of acid and gas is a direct result of the fermentation of glucose, as seen with unknown

Artemia salina are used as environmental indicator species. They are used to test changes in their environment. A. salina is sensitive to toxins, temperature, and drugs. For the experiment, cysts were put into different amounts of ethanol alcohol and their viability was measured. The hypothesis used was if there is more ethanol alcohol, then the viability of the brine shrimp is unfavorable. The brine shrimp were put into sixteen Petri dishes with the same amount of brine solution. Different amounts of ethanol alcohol were added to each Petri dish. After 168 hours, the brine shrimp were

In this experiment, the main objective was to synthesize a ketone from borneol via an oxidation reaction and secondly, to produce a secondary alcohol from camphor via a reduction reaction. Therefore, the hypothesis of this lab is that camphor will be produced in the oxidation reaction and isoborneol will be the product of the reduction reaction because of steric hindrance. For the oxidation step, a reflux will be done and then a microscale reflux for the reduction step. The products will be confirmed using Infrared spectroscopy, the chromic acid test, 2,4-DNP test and 13C NMR spectroscopy. The results of this

Our project is testing how various liquids affect the growing rates and growth of Magic Growing Capsules. We were inspired to perform this experiment because we had done a similar experiment in 6th grade science class working with growing capsules, and we were curious to find out more about how the substance affects the speed at which the capsules grow. The Magic Growing Capsules are made of a large polymer, which is a substance that has a molecular structure consisting chiefly or entirely of a large number of similar units bonded together. When the capsule is submerged in water, the polymer expands, causing the capsule to turn into a sponge animal.

Sponges are creatures that many people do really take about, but the truth is that they are fascinating. They are simple compared to many creatures on this planet, but such a simple creature can have intriguing ways to function in life. They have so many different structures that help collect food and allow water flow. Also, there are a few different morphologies that they can take advantage of to survive. One of the most important factor to a sponge’s survival is the water flow velocity to area ratio.

Life on this planet began with microorganisms. Through millions of years microorganisms have found ways to successfully adapt and survive. These adaptations have created a wide biodiversity, allowing them to basically populate in all places. Why are these microbes so important? Because they shape the history of our world. Some microbes can be deathly to humans while some others are favorable, for example, bacteria that lives in the gut of both humans and animals and helps during the process of digestion (Alfred Brown & Heidi Smith, 2006). Understanding these interactions help scientists to find ways to protect humans from potential deathly pathogens. In order to observe microbes, microscope proficiency and microorganisms’ identification are crucial skills in a microbiology lab. During this laboratory session, samples of environmental and human organisms were inoculated into two different rich media and incubated to their according temperature. After this, appropriate use and calibration of the microscope was performed. Lastly, morphology and size of different species of bacteria, algae, fungi and protozoan were recorded.