For this lab we will be using an enzyme to catalyze a reaction to speed up the process of turning 2H2O2 into 2H2O + O2. An enzyme is a specific protein molecule that catalyze a specific chemical reaction. The catalase enzyme will be used. Catalase is an enzyme that helps break down H2O2 (Peroxide). These enzymes can be found in your liver to help break down toxins.
Enzymes are affected by many environmental things such as temperature, pH, how much of the enzyme compared to substrate. Each enzyme is different in what it will work best in not all enzymes will work the best in the same environment. Because the enzyme we are using
(catalase) is a human enzyme I would venture to say that it will best react and the humans normal temperature and pH which is 37 degrees Celsius and around
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April 28th, 2015
Methods
In this experiment various temperatures were tested to see how the enzyme catalase was effected. Four new test tubes were taken out. Each one being filled with the same solution of
5mL of 3.0% Peroxide and 3mL of water. These were the controlled variables they were the same throughout the entire experiment. The test tubes were placed in their respective temperatures of 0, 23, 37, and 55 degrees Celsius. These are the independent variables the only thing that changed over the length of the experiment. Test tube 1 was placed in an ice bath to achieve 0 degrees Celsius this will be my control or base to compare the others off of, test tube 2 was kept at room temperature 23 degrees Celsius, test tube 3 and 4 were placed in water baths that had been prepared to achieve there temperatures at 37 and 55 degrees Celsius. They were left in the respective temperatures for ten minutes before moving to the next step to ensure that each solution was at the proper temperature. The next step was performed the same on each test tube one at a time only change was the temperature of the test tube. On each test tube 10 drops
Of the thousands of enzymes known, there is a family of enzymes called proteases that catalyze a reaction of breaking down proteins. What do you think would happen if you added a protease to your sample of catalase before proceeding with your experiment?
In this above reaction, oxygen is released and is used for other cellular purposes, but when it occurs in a test tube, similar to this experiment, the oxygen gas bubbles producing a layer of foam on the surface of the peroxide. The amount of foam and the speed it is produced are forms of measuring the catalase activity. In the next experiments, one would determine the degree of catalase action by calculating the thickness of the foam layer. It is hypothesized that when reacting with: potato, apple, steak, or liver, the plants and animal tissues will react differently.
Enzymes are biological catalysts, which means it decreases activation energy in reactions. The lower activation energy in a reaction, the faster the reaction rate. Many enzymes alter their shape when they bind to the activation site. This is called induced fit, meaning for the enzyme to work to its full potential it has to change shape to binding substrate. The location of enzyme’s activation site is on the surface of the enzyme, where the binding of substrates take place. Enzyme activity can be influenced by a variety of environmental factors. If the concentration of enzyme is low, and there is a great deal of substrate, then increasing enzyme concentration results in more molecules available to convert substrates to products. Thus, increasing enzyme concentration can increase reaction rate. If substrate concentrations are low, and many of the existing enzymes are idle because of a lack of substrate, then adding enzyme will have no effect on reaction rate. Enzyme concentration affects the enzyme activity, because the more enzyme concentration the faster the reaction rate, until it hits it’s limiting factor. When substrate concentration is increased, it also increases rate of reaction. Temperature plays an important
Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a 'ball' shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, a product is produced. Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. It is said that the substrate is complimentary to their substrate.
13. The temperature of the water was measured prior to the tube being placed in it and the temperature of the Hydrochloric Acid was measured after it 's temperature had adjusted.
3. We poured tube 1 with the solution in tube 3 to combine them. We repeated this for all of the tubes. Each of the tubes in step 1 was mixed with a tube in step 3, making there be 6 total test tubes with a solution in it.
Place the beaker on the hot plate, place the thermometer in the beaker and set the hot plate to 5oC.
The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,
As seen on table 1, the hypothesis in the introduction of this lab has been supported by the procedures. As the temperature varied from catalases optimal of 37° C, the reaction rate of catalase decreased. 37°C had the highest reaction rate of the three, at 3, while 4°C had the middle rate of reaction at 2.5, and 100° C had the lowest reaction rate of 0.5.
We increased the substrate, catechol, while maintaining the enzyme, catecholase, at the same volume. We came to the hypothesis that if the substrate was increased, then the rate of reaction would increase as long as there is enzymes to react with. The third experiment involved how temperature would affect enzyme activity. This was done by placing certain vials in certain temperatures and then analyzing the data in the spectrophotometer. We came to the hypothesis that if the temperature was increased, then the reaction rate would increase until it reaches the optimal temperature after that the reaction rate would decrease. This is because the enzyme will start to denature if the temperatures get too high or too low. The fourth experiment deals with how pH affects enzyme activity. For this experiment different vials with catecholase and different pH’s were observed under a spectrophotometer to see how the enzyme would be affected. We found our hypothesis to be, if pH is raised, then the reaction rate will increase until it reaches the optimal pH. The reaction rate will increase until the optimal pH because after the optimal pH, the enzyme will start to denature and not function as it
During the lab various factors were changed in order to compare how different environmental changes affected the rate of the reaction. A first test was conducted with liver and hydrogen peroxide without an induced temperature change scored a 5. Next, the catalase (liver) was heated to a high temperature and then hydrogen peroxide was added. The reaction was not as fast, and produced a score of 3. A similar test was done using another piece of liver but was placed in an ice bath and then hydrogen peroxide was added. This produced an even slower reaction rate of 2. These three tests demonstrate how temperature greatly influences the rate of enzyme action. When enzymes reach above boiling point, they are denatured and no longer function. Optimal temperatures for enzymes to function is 35-40 degrees Celsius. When the temperature is lower than optimal, slow reactions occur. Further experiments were conducted to show how pH levels affected enzyme action. Two mL of hydrogen peroxide was added to three test tubes. Then, HCl was added to test tube 1, NaOH was
However extreme temperatures, for example, boiling water, will result in inconclusive data with no effect on the reaction rate. The purpose of this experiment is to measure the reaction rate of the catalase when interacting with different temperatures and substrate concentrations. This is significant because we can test and compare the timings of different factors that will affect the catalase reaction rate
Organisms cannot depend solely on spontaneous reactions for the production of materials because they occur slowly and are not responsive to the organism's needs (Martineau, Dean, et al, Laboratory Manual, 43). In order to speed up the reaction process, cells use enzymes as biological catalysts. Enzymes are able to speed up the reaction through lowering activation energy. Additionally, enzymes facilitate reactions without being consumed (manual,43). Each enzyme acts on a specific molecule or set of molecules referred to as the enzyme's substrate and the results of this reaction are called products (manual 43). As a result, enzymes promote a reaction so that substrates are converted into products on a faster pace (manual 43). Most enzymes are proteins whose structure is determined by its sequence of its amino acids. Enzymes are designed to function the best under physiological conditions of PH and temperature. Any change of these variables that change the conformation of the enzyme will destroy or enhance enzyme activity(manual, 43).
The purpose of this experiment was to test the effects that temperature, pH, and substrate
What are the effects of different temperatures on the decomposition of Hydrogen Peroxide with the Enzyme Catalase. Different enzymes work best at different optimal temperatures, and with this experiment we hope to discover which temperatures are unideal for Catalase. The substrate is H2O2 which binds to the active site of the enzyme Catalase. The reaction in question is as written below: