Cinderella Lab Report

Decent Essays

The primary goal of this investigation was to determine whether bacteria commonly isolated as a pair from contact lens cases could coaggregate or cohere. The organisms were used in this study was isolated from the contact lens cases of asymptomatic daily wearers and stored in culture stock. The primary outcome of this study demonstrated that coaggregation and cohesion occurred between Acinetobacter spp, Staphylococcus spp and Micrococcus spp. Two Gram positive bacteria, Staphylococcus spp and Micrococcus spp also showed coaggregation. But, Acinetobacter spp and Micrococcus spp showed sporadic coaggregation. However, no coaggregating pairs exhibited the high scores given by the positive control paring of A. naeslundii and S. sanguinis, which …show more content…

Indeed, this study showed the evidence that when Staphylococcus spp grown together with Acinetobacter spp or Micrococcus spp, could influence the growth of Staphylococcus spp. Additionally, Staphylococci influenced the growth of Acinetobacter spp, but mixed response was seen with Micrococcus spp.
Swartz et al (1987) demonstrated coaggregation and cohesion with dental plaque bacteria, where Actinomyces viscosus foster the biofilm establishment of Bacteroides gingivalis on teeth by enhancing its adherence. Similarly, the present study finding showed that the contact lens case isolates, Micrococcus spp and Acinetobacter spp can influenced the adherence of Staphylococcus spp in the contact case surface. Staphylococcus spp. identified as the most commonly from the contact lens cases in asymptomatic wearers, also reported as the most commonly found as a part of the normal ocular flora (eye lid, conjunctiva) or extra-ocular microbiota (facial) and elsewhere on human body8,14 Along …show more content…

Thus we sought to determine whether those bacteria that produced coaggregation were associated with cohesion in contact lens case biofilm formation. Cohesion can also occur when a pioneer species modifies the substratum to encourage adhesion of successor species. As, Staphylococcus spp are the most commonly isolated from the lens cases, could be considered as pioneer species, but 10% of the lens cases also showed the contamination of Gram negative bacteria. Based on recent in vivo data we found Staphylococcus spp often isolated with Acinetobacter spp. There is other literature which also showed the isolation of P. aeruginosa and S. marcescens from lens cases. The present study showed cohesion between Staphylococcus spp and Acinetobacter spp/ Micrococcus spp, where Staphylococcus spp can be considered as pioneer species and Acinetobacter and Micrococcus as successor. Whist this study did not investigate the facilitating factor for the cohesion, will be worth to consider in future study. The next step will be to see whether the elimination of Micrococcus and Acinetobacter contamination from lens cases will reduce the adhesion of Staphylococcus spp which may reduce the overall rate of contact lens case

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